Enzymatic dynamic kinetic resolution of racemic N -formyl- and N -carbamoyl-amino acids using immobilized l - N -carbamoylase and N -succinyl-amino acid racemase
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| 024 | 7 | 0 | |a 10.1007/s00253-014-5880-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-5880-7 | ||
| 245 | 0 | 0 | |a Enzymatic dynamic kinetic resolution of racemic N -formyl- and N -carbamoyl-amino acids using immobilized l - N -carbamoylase and N -succinyl-amino acid racemase |h [Elektronische Daten] |c [Pablo Soriano-Maldonado, Francisco Las Heras-Vazquez, Josefa Clemente-Jimenez, Felipe Rodriguez-Vico, Sergio Martínez-Rodríguez] |
| 520 | 3 | |a Taking advantage of the catalytic promiscuity of l-carbamoylase from Geobacillus stearothermophilus CECT43 (BsLcar) and N-succinyl-amino acid racemase from Geobacillus kaustophilus CECT4264 (GkNSAAR), we have evaluated the production of different optically pure l-α-amino acids starting from different racemic N-formyl- and N-carbamoyl-amino acids using a dynamic kinetic resolution approach. The enzymes were immobilized on two different solid supports, resulting in improved stability of the enzymes in terms of thermostability and storage when compared to the enzymes in solution. The bienzymatic system retained up to 80% conversion efficiency after 20weeks at 4°C and up to 90% after 1week at 45°C. The immobilization process also resulted in a great enhancement of the activity of BsLcar toward N-formyl-tryptophan, showing for the first time that substrate specificity of l-carbamoylases can be influenced by this approach. The system was effective for the biosynthesis of natural and unnatural l-amino acids (enantiomeric excess (e.e.) >99.5%), such as l-methionine, l-alanine, l-tryptophan, l-homophenylalanine, l-aminobutyric acid, and l-norleucine, with a higher performance toward N-formyl-α-amino acid substrates. Biocatalyst reuse was studied, and after 10 reaction cycles, over 75% activity remained. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a l - N -Carbamoylase |2 nationallicence | |
| 690 | 7 | |a N -Succinyl-amino acid racemase |2 nationallicence | |
| 690 | 7 | |a N -Acetyl-amino acid racemase |2 nationallicence | |
| 690 | 7 | |a l -Norleucine |2 nationallicence | |
| 690 | 7 | |a l -Homophenylalanine |2 nationallicence | |
| 690 | 7 | |a l -2-Aminobutyric acid |2 nationallicence | |
| 690 | 7 | |a l -Tryptophan |2 nationallicence | |
| 700 | 1 | |a Soriano-Maldonado |D Pablo |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | |
| 700 | 1 | |a Las Heras-Vazquez |D Francisco |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | |
| 700 | 1 | |a Clemente-Jimenez |D Josefa |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | |
| 700 | 1 | |a Rodriguez-Vico |D Felipe |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | |
| 700 | 1 | |a Martínez-Rodríguez |D Sergio |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 283-291 |x 0175-7598 |q 99:1<283 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-5880-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-5880-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Soriano-Maldonado |D Pablo |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Las Heras-Vazquez |D Francisco |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Clemente-Jimenez |D Josefa |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rodriguez-Vico |D Felipe |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Martínez-Rodríguez |D Sergio |u Departamento de Química y Física, Universidad de Almería, Campus de Excelencia Internacional Agroalimentario, CEIA3, Edificio CITE I, Carretera de Sacramento s/n., 04120 La Cañada de San Urbano, Almería, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 283-291 |x 0175-7598 |q 99:1<283 |1 2015 |2 99 |o 253 | ||