Fast and highly specific DNA-based multiplex detection on a solid support
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605498091 | ||
| 003 | CHVBK | ||
| 005 | 20210128100544.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6246-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6246-x | ||
| 245 | 0 | 0 | |a Fast and highly specific DNA-based multiplex detection on a solid support |h [Elektronische Daten] |c [Ivan Barišić, Verena Kamleithner, Silvia Schönthaler, Herbert Wiesinger-Mayr] |
| 520 | 3 | |a Highly specific and fast multiplex detection methods are essential to conduct reasonable DNA-based diagnostics and are especially important to characterise infectious diseases. More than 1000 genetic targets such as antibiotic resistance genes, virulence factors and phylogenetic markers have to be identified as fast as possible to facilitate the correct treatment of a patient. In the present work, we developed a novel ligation-based DNA probe concept that was combined with the microarray technology and used it for the detection of bacterial pathogens. The novel linear chain (LNC) probes identified all tested species correctly within 1h based on their 16S rRNA gene in a 25-multiplex reaction. Genomic DNA was used directly as template in the ligation reaction identifying as little as 107 cells without any pre-amplification. The high specificity was further demonstrated characterising a single nucleotide polymorphism leading to no false positive fluorescence signals of the untargeted single nucleotide polymorphism (SNP) variants. In comparison to conventional microarray probes, the sensitivity of the novel LNC3 probes was higher by a factor of 10 or more. In summary, we present a fast, simple, highly specific and sensitive multiplex detection method adaptable for a wide range of applications. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Molecular biology |2 nationallicence | |
| 690 | 7 | |a DNA microarray |2 nationallicence | |
| 690 | 7 | |a Solid support-based ligation reaction |2 nationallicence | |
| 690 | 7 | |a Multiplex detection |2 nationallicence | |
| 690 | 7 | |a Pathogen characterisation |2 nationallicence | |
| 700 | 1 | |a Barišić |D Ivan |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | |
| 700 | 1 | |a Kamleithner |D Verena |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | |
| 700 | 1 | |a Schönthaler |D Silvia |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | |
| 700 | 1 | |a Wiesinger-Mayr |D Herbert |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 413-423 |x 0175-7598 |q 99:1<413 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6246-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6246-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Barišić |D Ivan |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kamleithner |D Verena |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schönthaler |D Silvia |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wiesinger-Mayr |D Herbert |u Molecular Diagnostics, AIT Austrian Institute of Technology, Muthgasse 11, 1190, Vienna, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 413-423 |x 0175-7598 |q 99:1<413 |1 2015 |2 99 |o 253 | ||