caa a22 4500 605498105 CHVBK 20210128100544.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6219-0 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6219-0 Critical factors affecting laccase-mediated biobleaching of pulp in paper industry [Elektronische Daten] [Gursharan Singh, Kavleen Kaur, Sanjeev Puri, Prince Sharma] Next to xylanases, laccases from fungi and alkali-tolerant bacteria are the most important biocatalysts that can be employed for eco-friendly biobleaching of hard and soft wood pulps in the paper industry. Laccases offer a potential alternative to conventional, environmental-polluting chlorine and chlorine-based bleaching and has no reductive effect on the final yield of pulp as compared to hemicellulases (xylanases and mannanases). In the last decade, reports on biobleaching with laccases are based on laboratory observations only. There are several critical challenges before this enzyme can be implemented for pulp bleaching at the industrial scale. This review discusses significant factors like redox potential, laccase mediator system (LMS)—synthetic or natural, pH, temperature, stability of enzyme, unwanted grafting reactions of laccase, and cost-intensive production at large scale which constitute a great hitch for the successful implementation of laccases at industrial level. Springer-Verlag Berlin Heidelberg, 2014 Laccase nationallicence Biobleaching nationallicence Redox potential nationallicence Grafting nationallicence pH nationallicence Mediators nationallicence Singh Gursharan Biotechnology Branch, University Institute of Engineering and Technology, Panjab University, Chandigarh, India aut Kaur Kavleen Biotechnology Branch, University Institute of Engineering and Technology, Panjab University, Chandigarh, India aut Puri Sanjeev Biotechnology Branch, University Institute of Engineering and Technology, Panjab University, Chandigarh, India aut Sharma Prince Department of Microbiology, Panjab University, Chandigarh, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 155-164 0175-7598 99:1<155 2015 99 253 https://doi.org/10.1007/s00253-014-6219-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6219-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Gursharan Biotechnology Branch, University Institute of Engineering and Technology, Panjab University, Chandigarh, India aut NATIONALLICENCE 700 1- Kaur Kavleen Biotechnology Branch, University Institute of Engineering and Technology, Panjab University, Chandigarh, India aut NATIONALLICENCE 700 1- Puri Sanjeev Biotechnology Branch, University Institute of Engineering and Technology, Panjab University, Chandigarh, India aut NATIONALLICENCE 700 1- Sharma Prince Department of Microbiology, Panjab University, Chandigarh, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 155-164 0175-7598 99:1<155 2015 99 253