caa a22 4500 605498121 CHVBK 20210128100544.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6076-x doi (NATIONALLICENCE)springer-10.1007/s00253-014-6076-x Phenotype and expression profile analysis of Staphylococcus aureus biofilms and planktonic cells in response to licochalcone A [Elektronische Daten] [Fengge Shen, Xudong Tang, Yang Wang, Zhiqiang Yang, Xiaochen Shi, Chao Wang, Qiaoli Zhang, Yanan An, Wei Cheng, Kunqi Jin, Mingyuan Liu, Na Guo, Lu Yu] Staphylococcus aureus is one of the most important pathogens in humans and animals. The formation of biofilm by S. aureus is considered an important mechanism of antimicrobial resistance. Therefore, finding effective drugs against the biofilm produced by S. aureus has been a high priority. Licochalcone A (LAA), a natural plant product, was reported to have antibacterial activities and showed good activity against all 21 tested strains of S. aureus biofilm and planktonic cells. To detect the possible molecular mechanism of LAA against S. aureus biofilm or planktonic cells, Affymetrix GeneChips were used to determine the global comparative transcription of S. aureus biofilm and planktonic cells triggered by treatment with sub-bactericidal and sub-inhibitory concentrations of LAA, respectively. LAA significantly altered (greater than a 2- or less than −2-fold change) the expression of 693 genes in planktonic cells and 817 genes in biofilm. The levels of genes encoding autolysis-associated proteins, cell wall proteins, pathogenic factors, protein synthesis genes, and enzymes involved in capsule synthesis were significantly altered in LAA-treated S. aureus. Furthermore, some differences observed in the microarray analysis were verified by real-time RT-PCR. To our knowledge, this is the first observation of phenotype and expression profiles of S. aureus biofilm and planktonic cells in response to LAA treatment. Springer-Verlag Berlin Heidelberg, 2014 Staphylococcus aureus nationallicence Licochalcone A nationallicence Biofilm nationallicence GeneChip nationallicence Transcription nationallicence Shen Fengge Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Tang Xudong Key Lab for New Drug Research of TCM, Research Institute of Tsinghua University in Shenzhen, Shenzhen, China aut Wang Yang Department of Infectious Diseases, First Hospital of Jilin University, Changchun, China aut Yang Zhiqiang Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Shi Xiaochen Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Wang Chao Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Zhang Qiaoli Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut An Yanan Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Cheng Wei Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Jin Kunqi Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Liu Mingyuan Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Guo Na Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Yu Lu Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 359-373 0175-7598 99:1<359 2015 99 253 https://doi.org/10.1007/s00253-014-6076-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6076-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Fengge Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Tang Xudong Key Lab for New Drug Research of TCM, Research Institute of Tsinghua University in Shenzhen, Shenzhen, China aut NATIONALLICENCE 700 1- Wang Yang Department of Infectious Diseases, First Hospital of Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Yang Zhiqiang Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Shi Xiaochen Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Wang Chao Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Zhang Qiaoli Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- An Yanan Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Cheng Wei Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Jin Kunqi Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Liu Mingyuan Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Guo Na Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 700 1- Yu Lu Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 359-373 0175-7598 99:1<359 2015 99 253