Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6082-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6082-z | ||
| 245 | 0 | 0 | |a Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli |h [Elektronische Daten] |c [Selma Maric, Mikkel Thygesen, Jürgen Schiller, Magdalena Marek, Martine Moulin, Michael Haertlein, V. Forsyth, Mikhail Bogdanov, William Dowhan, Lise Arleth, Thomas Pomorski] |
| 520 | 3 | |a Phosphatidylcholine (PC) is a major component of eukaryotic cell membranes and one of the most commonly used phospholipids for reconstitution of membrane proteins into carrier systems such as lipid vesicles, micelles and nanodiscs. Selectively deuterated versions of this lipid have many applications, especially in structural studies using techniques such as NMR, neutron reflectivity and small-angle neutron scattering. Here we present a comprehensive study of selective deuteration of phosphatidylcholine through biosynthesis in a genetically modified strain of Escherichia coli. By carefully tuning the deuteration level in E. coli growth media and varying the deuteration of supplemented carbon sources, we show that it is possible to achieve a controlled deuteration for three distinct parts of the PC lipid molecule, namely the (a) lipid head group, (b) glycerol backbone and (c) fatty acyl tail. This biosynthetic approach paves the way for the synthesis of specifically deuterated, physiologically relevant phospholipid species which remain difficult to obtain through standard chemical synthesis. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Glycerophospholipids |2 nationallicence | |
| 690 | 7 | |a Phosphatidylcholine |2 nationallicence | |
| 690 | 7 | |a Selective deuteration |2 nationallicence | |
| 690 | 7 | |a Biosynthesis |2 nationallicence | |
| 690 | 7 | |a E. coli |2 nationallicence | |
| 690 | 7 | |a Neutron scattering |2 nationallicence | |
| 690 | 7 | |a NMR |2 nationallicence | |
| 690 | 7 | |a Mass spectrometry |2 nationallicence | |
| 690 | 7 | |a Evolution in the test tube |2 nationallicence | |
| 700 | 1 | |a Maric |D Selma |u Structural Biophysics, Niels Bohr Institute, Faculty of Science, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark |4 aut | |
| 700 | 1 | |a Thygesen |D Mikkel |u CARB Centre, Department of Chemistry, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark |4 aut | |
| 700 | 1 | |a Schiller |D Jürgen |u Institut für Medizinische Physik und Biophysik, Medizinische Fakultät, Universität Leipzig, Härtelstrasse 16-18, 04107, Leipzig, Germany |4 aut | |
| 700 | 1 | |a Marek |D Magdalena |u Center for Membrane Pumps in Cells and Disease, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark |4 aut | |
| 700 | 1 | |a Moulin |D Martine |u Life Sciences Group, Institut Laue Langevin, 6 rue Jules Horowitz, CEDEX 9, BP156, 38042, Grenoble, France |4 aut | |
| 700 | 1 | |a Haertlein |D Michael |u Life Sciences Group, Institut Laue Langevin, 6 rue Jules Horowitz, CEDEX 9, BP156, 38042, Grenoble, France |4 aut | |
| 700 | 1 | |a Forsyth |D V. |u Life Sciences Group, Institut Laue Langevin, 6 rue Jules Horowitz, CEDEX 9, BP156, 38042, Grenoble, France |4 aut | |
| 700 | 1 | |a Bogdanov |D Mikhail |u Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, 77030, Houston, TX, USA |4 aut | |
| 700 | 1 | |a Dowhan |D William |u Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, 77030, Houston, TX, USA |4 aut | |
| 700 | 1 | |a Arleth |D Lise |u Structural Biophysics, Niels Bohr Institute, Faculty of Science, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark |4 aut | |
| 700 | 1 | |a Pomorski |D Thomas |u Center for Membrane Pumps in Cells and Disease, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 241-254 |x 0175-7598 |q 99:1<241 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6082-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6082-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Maric |D Selma |u Structural Biophysics, Niels Bohr Institute, Faculty of Science, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Thygesen |D Mikkel |u CARB Centre, Department of Chemistry, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schiller |D Jürgen |u Institut für Medizinische Physik und Biophysik, Medizinische Fakultät, Universität Leipzig, Härtelstrasse 16-18, 04107, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marek |D Magdalena |u Center for Membrane Pumps in Cells and Disease, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Moulin |D Martine |u Life Sciences Group, Institut Laue Langevin, 6 rue Jules Horowitz, CEDEX 9, BP156, 38042, Grenoble, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Haertlein |D Michael |u Life Sciences Group, Institut Laue Langevin, 6 rue Jules Horowitz, CEDEX 9, BP156, 38042, Grenoble, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Forsyth |D V. |u Life Sciences Group, Institut Laue Langevin, 6 rue Jules Horowitz, CEDEX 9, BP156, 38042, Grenoble, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bogdanov |D Mikhail |u Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, 77030, Houston, TX, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dowhan |D William |u Department of Biochemistry and Molecular Biology, University of Texas Medical School at Houston, 77030, Houston, TX, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Arleth |D Lise |u Structural Biophysics, Niels Bohr Institute, Faculty of Science, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pomorski |D Thomas |u Center for Membrane Pumps in Cells and Disease, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 241-254 |x 0175-7598 |q 99:1<241 |1 2015 |2 99 |o 253 | ||