caa a22 4500 605498148 CHVBK 20210128100544.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6217-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6217-2 Modifying the lipid content and composition of plant seeds: engineering the production of LC-PUFA [Elektronische Daten] [Noemi Ruiz-Lopez, Sarah Usher, Olga Sayanova, Johnathan Napier, Richard Haslam] Omega-3 fatty acids are characterized by a double bond at the third carbon atom from the end of the carbon chain. Latterly, long chain polyunsaturated omega-3 fatty acids such as eicosapentaenoic acid (EPA; 20:5Δ5,8,11,14,17) and docosahexanoic acid (DHA; 22:6 Δ4,7,10,13,16,19), which typically only enter the human diet via the consumption of oily fish, have attracted much attention. The health benefits of the omega-3 LC-PUFAs EPA and DHA are now well established. Given the desire for a sustainable supply of omega-LC-PUFA, efforts have focused on enhancing the composition of vegetable oils to include these important fatty acids. Specifically, EPA and DHA have been the focus of much study, with the ultimate goal of producing a terrestrial plant-based source of these so-called fish oils. Over the last decade, many genes encoding the primary LC-PUFA biosynthetic activities have been identified and characterized. This has allowed the reconstitution of the LC-PUFA biosynthetic pathway in oilseed crops, producing transgenic plants engineered to accumulate omega-3 LC-PUFA to levels similar to that found in fish oil. In this review, we will describe the most recent developments in this field and the challenges of overwriting endogenous seed lipid metabolism to maximize the accumulation of these important fatty acids. The Author(s), 2014 Omega-3 fatty acids nationallicence Polyunsaturated fatty acid nationallicence Triacylglycerol nationallicence Transgenic plant nationallicence Oilseed nationallicence Camelina sativa nationallicence Ruiz-Lopez Noemi Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut Usher Sarah Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut Sayanova Olga Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut Napier Johnathan Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut Haslam Richard Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 143-154 0175-7598 99:1<143 2015 99 253 https://doi.org/10.1007/s00253-014-6217-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6217-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruiz-Lopez Noemi Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut NATIONALLICENCE 700 1- Usher Sarah Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut NATIONALLICENCE 700 1- Sayanova Olga Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut NATIONALLICENCE 700 1- Napier Johnathan Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut NATIONALLICENCE 700 1- Haslam Richard Department of Biological Chemistry, Rothamsted Research, AL5 2JQ, Harpenden, Herts, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 143-154 0175-7598 99:1<143 2015 99 253