caa a22 4500 605498237 CHVBK 20210128100545.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6729-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6729-4 Microbially influenced corrosion communities associated with fuel-grade ethanol environments [Elektronische Daten] [Charles Williamson, Luke Jain, Brajendra Mishra, David Olson, John Spear] Microbially influenced corrosion (MIC) is a costly problem that impacts hydrocarbon production and processing equipment, water distribution systems, ships, railcars, and other types of metallic infrastructure. In particular, MIC is known to cause considerable damage to hydrocarbon fuel infrastructure including production, transportation, and storage systems, often times with catastrophic environmental contamination results. As the production and use of alternative fuels such as fuel-grade ethanol (FGE) increase, it is important to consider MIC of engineered materials exposed to these "newer fuels” as they enter existing infrastructure. Reports of suspected MIC in systems handling FGE and water prompted an investigation of the microbial diversity associated with these environments. Small subunit ribosomal RNA gene pyrosequencing surveys indicate that acetic-acid-producing bacteria (Acetobacter spp. and Gluconacetobacter spp.) are prevalent in environments exposed to FGE and water. Other microbes previously implicated in corrosion, such as sulfate-reducing bacteria and methanogens, were also identified. In addition, acetic-acid-producing microbes and sulfate-reducing microbes were cultivated from sampled environments containing FGE and water. Results indicate that complex microbial communities form in these FGE environments and could cause significant MIC-related damage that may be difficult to control. How to better manage these microbial communities will be a defining aspect of improving mitigation of global infrastructure corrosion. The Author(s), 2015 Microbial diversity nationallicence Fuel-grade ethanol nationallicence Microbiologically influenced corrosion nationallicence Pyrosequencing nationallicence Williamson Charles Department of Civil and Environmental Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut Jain Luke Department of Metallurgy and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut Mishra Brajendra Department of Metallurgy and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut Olson David Department of Metallurgy and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut Spear John Department of Civil and Environmental Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6945-6957 0175-7598 99:16<6945 2015 99 253 https://doi.org/10.1007/s00253-015-6729-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6729-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Williamson Charles Department of Civil and Environmental Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut NATIONALLICENCE 700 1- Jain Luke Department of Metallurgy and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut NATIONALLICENCE 700 1- Mishra Brajendra Department of Metallurgy and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut NATIONALLICENCE 700 1- Olson David Department of Metallurgy and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut NATIONALLICENCE 700 1- Spear John Department of Civil and Environmental Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6945-6957 0175-7598 99:16<6945 2015 99 253