caa a22 4500 605498245 CHVBK 20210128100545.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6781-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6781-0 Archaeal DNA polymerases in biotechnology [Elektronische Daten] [Likui Zhang, Manyu Kang, Jiajun Xu, Yanchao Huang] DNA polymerase (pol) is a ubiquitous enzyme that synthesizes DNA strands in all living cells. In vitro, DNA pol is used for DNA manipulation, including cloning, PCR, site-directed mutagenesis, sequencing, and several other applications. Family B archaeal DNA pols have been widely used for molecular biological methods. Biochemical and structural studies reveal that each archaeal DNA pol has different characteristics with respect to fidelity, processivity and thermostability. Due to their high fidelity and strong thermostability, family B archaeal DNA pols have the extensive application on high-fidelity PCR, DNA sequencing, and site-directed mutagenesis while family Y archaeal DNA pols have the potential for error-prone PCR and random mutagenesis because of their low fidelity and strong thermostability. This information combined with mutational analysis has been used to construct novel DNA pols with altered properties that enhance their use as biotechnological reagents. In this review, we focus on the development and use of family B archaeal DNA pols. Springer-Verlag Berlin Heidelberg, 2015 Archaeal DNA pols nationallicence PCR nationallicence DNA sequencing nationallicence Site-directed mutagenesis nationallicence Fidelity nationallicence Processivity nationallicence Zhang Likui Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut Kang Manyu Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut Xu Jiajun Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut Huang Yanchao Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6585-6597 0175-7598 99:16<6585 2015 99 253 https://doi.org/10.1007/s00253-015-6781-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6781-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Likui Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut NATIONALLICENCE 700 1- Kang Manyu Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut NATIONALLICENCE 700 1- Xu Jiajun Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut NATIONALLICENCE 700 1- Huang Yanchao Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, 225127, Yangzhou City, Jiangsu Province, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6585-6597 0175-7598 99:16<6585 2015 99 253