caa a22 4500 605498288 CHVBK 20210128100545.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6564-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6564-7 Evidence for cholesterol-lowering activity by Bifidobacterium bifidum PRL2010 through gut microbiota modulation [Elektronische Daten] [Ilaria Zanotti, Francesca Turroni, Antonio Piemontese, Leonardo Mancabelli, Christian Milani, Alice Viappiani, Gilda Prevedini, Borja Sanchez, Abelardo Margolles, Lisa Elviri, Bernini Franco, Douwe van Sinderen, Marco Ventura] Bifidobacteria are members of the human gut microbiota, which are known to influence the metabolic abilities of their host. Here, we investigated the capabilities of bifidobacteria to reduce cholesterol levels in synthetic growth media, clearly demonstrating assimilation of this molecule by particular bifidobacterial strains, including Bifidobacterium bifidum PRL2010 (LMG S-28692). The transcriptomic analysis of PRL2010 cells cultivated in the presence of cholesterol revealed a significantly increased transcription level of genes encoding putative transporters and reductases, indicative of specific mechanisms for cholesterol assimilation as well as cholesterol conversion to coprostanol. Cholesterol lowering activity of B. bifidum PRL2010 cells was further evaluated by means of an in vivo murine model, showing that the fecal microbiota of mice is modified toward those bacteria involved in the metabolism of cholesterol. Springer-Verlag Berlin Heidelberg, 2015 Bifidobacterium nationallicence Cholesterol nationallicence Gut microbiota nationallicence Functional genomics nationallicence Zanotti Ilaria Department of Pharmacy, University of Parma, Parma, Italy aut Turroni Francesca Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland aut Piemontese Antonio Department of Pharmacy, University of Parma, Parma, Italy aut Mancabelli Leonardo Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut Milani Christian Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut Viappiani Alice GenProbio srl, Parma, Italy aut Prevedini Gilda Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut Sanchez Borja Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Villaviciosa, Asturias, Spain aut Margolles Abelardo Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Villaviciosa, Asturias, Spain aut Elviri Lisa Department of Pharmacy, University of Parma, Parma, Italy aut Franco Bernini Department of Pharmacy, University of Parma, Parma, Italy aut van Sinderen Douwe Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland aut Ventura Marco Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6813-6829 0175-7598 99:16<6813 2015 99 253 https://doi.org/10.1007/s00253-015-6564-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6564-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zanotti Ilaria Department of Pharmacy, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Turroni Francesca Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland aut NATIONALLICENCE 700 1- Piemontese Antonio Department of Pharmacy, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Mancabelli Leonardo Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Milani Christian Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Viappiani Alice GenProbio srl, Parma, Italy aut NATIONALLICENCE 700 1- Prevedini Gilda Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Sanchez Borja Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Villaviciosa, Asturias, Spain aut NATIONALLICENCE 700 1- Margolles Abelardo Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Villaviciosa, Asturias, Spain aut NATIONALLICENCE 700 1- Elviri Lisa Department of Pharmacy, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Franco Bernini Department of Pharmacy, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- van Sinderen Douwe Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland aut NATIONALLICENCE 700 1- Ventura Marco Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6813-6829 0175-7598 99:16<6813 2015 99 253