caa a22 4500 605498296 CHVBK 20210128100545.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6572-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6572-7 Self-organised nanoarchitecture of titanium surfaces influences the attachment of Staphylococcus aureus and Pseudomonas aeruginosa bacteria [Elektronische Daten] [Vi Truong, Vy Pham, Alexander Medvedev, Rimma Lapovok, Yuri Estrin, Terry Lowe, Vladimir Baulin, Veselin Boshkovikj, Christopher Fluke, Russell Crawford, Elena Ivanova] The surface nanotopography and architecture of medical implant devices are important factors that can control the extent of bacterial attachment. The ability to prevent bacterial attachment substantially reduces the possibility of a patient receiving an implant contracting an implant-borne infection. We now demonstrated that two bacterial strains, Staphylococcus aureus and Pseudomonas aeruginosa, exhibited different attachment affinities towards two types of molecularly smooth titanium surfaces each possessing a different nanoarchitecture. It was found that the attachment of S. aureus cells was not restricted on surfaces that had an average roughness (S a) less than 0.5nm. In contrast, P. aeruginosa cells were found to be unable to colonise surfaces possessing an average roughness below 1nm, unless sharp nanoprotrusions of approximately 20nm in size and spaced 35.0nm apart were present. It is postulated that the enhanced attachment of P. aeruginosa onto the surfaces possessing these nanoprotrusions was facilitated by the ability of the cell membrane to stretch over the tips of the nanoprotrusions as confirmed through computer simulation, together with a concomitant increase in the level of extracellular polymeric substance (EPS) being produced by the bacterial cells. Springer-Verlag Berlin Heidelberg, 2015 Bacterial attachment nationallicence Surface nanoarchitecture nationallicence Molecularly smooth surfaces nationallicence Staphylococcus aureus nationallicence Pseudomonas aeruginosa nationallicence Truong Vi School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut Pham Vy School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut Medvedev Alexander Centre for Advanced Hybrid Materials, Department of Materials Engineering, Monash University, 3800, Clayton, Victoria, Australia aut Lapovok Rimma Centre for Advanced Hybrid Materials, Department of Materials Engineering, Monash University, 3800, Clayton, Victoria, Australia aut Estrin Yuri Centre for Advanced Hybrid Materials, Department of Materials Engineering, Monash University, 3800, Clayton, Victoria, Australia aut Lowe Terry Department of Metallurgical and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut Baulin Vladimir Departament d'Enginyeria Quimica, Universitat Rovirai Virgili, 26 Avenue dels Paisos Catalans, 43007, Tarragona, Spain aut Boshkovikj Veselin School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut Fluke Christopher Centre for Astrophysics and Supercomputing, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut Crawford Russell School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut Ivanova Elena School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6831-6840 0175-7598 99:16<6831 2015 99 253 https://doi.org/10.1007/s00253-015-6572-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6572-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Truong Vi School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut NATIONALLICENCE 700 1- Pham Vy School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut NATIONALLICENCE 700 1- Medvedev Alexander Centre for Advanced Hybrid Materials, Department of Materials Engineering, Monash University, 3800, Clayton, Victoria, Australia aut NATIONALLICENCE 700 1- Lapovok Rimma Centre for Advanced Hybrid Materials, Department of Materials Engineering, Monash University, 3800, Clayton, Victoria, Australia aut NATIONALLICENCE 700 1- Estrin Yuri Centre for Advanced Hybrid Materials, Department of Materials Engineering, Monash University, 3800, Clayton, Victoria, Australia aut NATIONALLICENCE 700 1- Lowe Terry Department of Metallurgical and Materials Engineering, Colorado School of Mines, 80401, Golden, CO, USA aut NATIONALLICENCE 700 1- Baulin Vladimir Departament d'Enginyeria Quimica, Universitat Rovirai Virgili, 26 Avenue dels Paisos Catalans, 43007, Tarragona, Spain aut NATIONALLICENCE 700 1- Boshkovikj Veselin School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut NATIONALLICENCE 700 1- Fluke Christopher Centre for Astrophysics and Supercomputing, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut NATIONALLICENCE 700 1- Crawford Russell School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut NATIONALLICENCE 700 1- Ivanova Elena School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria, Australia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6831-6840 0175-7598 99:16<6831 2015 99 253