caa a22 4500 60549830X CHVBK 20210128100545.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6728-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6728-5 Transcriptional remodeling in response to transfer upon carbon-limited or metformin-supplemented media in S. cerevisiae and its effect on chronological life span [Elektronische Daten] [Esra Borklu-Yucel, Serpil Eraslan, Kutlu Ulgen] One of the factors affecting chronological life span (CLS) in budding yeast is nutrient, especially carbon limitation. Aside from metabolites in the growth medium such as glucose, amino acids, and acetic acid, many pharmaceuticals have also been proven to alter CLS. Besides their impact on life span, these drugs are also prospective chemicals to treat the age-associated diseases, so the identification of their action mechanism and their potential side effects is of crucial importance. In this study, the effects of caloric restriction and metformin, a dietary mimetic pharmaceutical, on yeast CLS are compared. Saccharomyces cerevisiae cells grown in synthetic dextrose complete (SDC) up to mid-exponential phase were either treated with metformin or were subjected to glucose limitation. The impacts of these perturbations were analyzed via transcriptomics, and the common (stimulation of glucose uptake, induction of mitochondrial maintenance, and reduction of protein translation) and divergent (stimulation of aerobic respiration and reprogramming of respiratory electron transport chain (ETC)) cellular responses specific to each treatment were determined. These results revealed that both glucose limitation and metformin treatment stimulate CLS extension and involve the mitochondrial function, probably by creating an efficient mitochondria-to-nucleus signaling of either aerobic respiration or ETC signaling stimulation, respectively. Springer-Verlag Berlin Heidelberg, 2015 Metformin nationallicence Carbon limitation nationallicence S. cerevisiae nationallicence Transcriptomics nationallicence Aerobic respiration nationallicence ETC nationallicence Borklu-Yucel Esra Department of Chemical Engineering, Bogazici University, Bebek, Istanbul, Turkey aut Eraslan Serpil Department of Chemical Engineering, Bogazici University, Bebek, Istanbul, Turkey aut Ulgen Kutlu Department of Chemical Engineering, Bogazici University, Bebek, Istanbul, Turkey aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6775-6789 0175-7598 99:16<6775 2015 99 253 https://doi.org/10.1007/s00253-015-6728-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6728-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borklu-Yucel Esra Department of Chemical Engineering, Bogazici University, Bebek, Istanbul, Turkey aut NATIONALLICENCE 700 1- Eraslan Serpil Department of Chemical Engineering, Bogazici University, Bebek, Istanbul, Turkey aut NATIONALLICENCE 700 1- Ulgen Kutlu Department of Chemical Engineering, Bogazici University, Bebek, Istanbul, Turkey aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6775-6789 0175-7598 99:16<6775 2015 99 253