caa a22 4500 605498326 CHVBK 20210128100545.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6581-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6581-6 Improvement in the UV resistance of baculoviruses by displaying nano-zinc oxide-binding peptides on the surfaces of their occlusion bodies [Elektronische Daten] [Jin Li, Yin Zhou, Chengfeng Lei, Wei Fang, Xiulian Sun] The sensitivity of baculoviruses to UV radiation severely limits their large-scale application as biological insecticides. The polyhedron envelope of a baculovirus, which is composed of carbohydrate and polyhedron envelope protein (PEP), is a significant structure for the stability and persistence of occlusion bodies (OBs) under environmental conditions. The results of this study revealed that the rough pitted surface phenotype of a pep-null Autographa californica multiple nucleopolyhedrovirus (AcMNPV) could not be rescued by any of its homologues, such as Helicoverpa armigera nucleopolyhedrovirus pep or Cydia pomonella granulovirus putative peps. In contrast, the N-terminal and middle flexible region (NM region, 1-167aa) of AcMNPV PEP were able to form an intact OB envelope. Furthermore, this region was capable of carrying eGFP to the surfaces of the OBs. To improve the UV resistance of AcMNPV OBs, two peptides capable of specifically binding to nano-ZnO were separately fused to the NM region of PEP. Under laboratory conditions, infectivity of the recombinant viruses binding to nano-ZnO particles was about ninefold higher than that without the nano-ZnO particles after UV-B irradiation. Pot experiments revealed that the half-life of the recombinant baculovirus binding nano-ZnO particles was 3.3 ± 0.15days, which was significantly longer than that of the control virus (0.49 ± 0.06days). These results therefore represent a new approach for the protection the baculoviral insecticides against UV irradiation in the field. Springer-Verlag Berlin Heidelberg, 2015 AcMNPV nationallicence Polyhedron envelope nationallicence Nano-ZnO-binding peptides nationallicence UV resistance nationallicence Li Jin Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut Zhou Yin Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut Lei Chengfeng Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut Fang Wei Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut Sun Xiulian Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6841-6853 0175-7598 99:16<6841 2015 99 253 https://doi.org/10.1007/s00253-015-6581-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6581-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jin Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhou Yin Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Lei Chengfeng Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Fang Wei Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Sun Xiulian Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, Hubei, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6841-6853 0175-7598 99:16<6841 2015 99 253