caa a22 4500 605498334 CHVBK 20210128100545.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6779-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6779-7 PEG-salt aqueous two-phase systems: an attractive and versatile liquid-liquid extraction technology for the downstream processing of proteins and enzymes [Elektronische Daten] [Anna Glyk, Thomas Scheper, Sascha Beutel] Nowadays, there is an increasing demand to establish new feasible, efficient downstream processing (DSP) techniques in biotechnology and related fields. Although several conventional DSP technologies have been widely employed, they are usually expensive and time-consuming and often provide only low recovery yields. Hence, the DSP is one major bottleneck for the commercialization of biological products. In this context, polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) represent a promising, efficient liquid-liquid extraction technology for the DSP of various biomolecules, such as proteins and enzymes. Furthermore, ATPS can overcome the limitations of traditional DSP techniques and have gained importance for applications in several fields of biotechnology due to versatile advantages over conventional DSP methods, such as biocompatibility, technical simplicity, and easy scale-up potential. In the present review, various practical applications of PEG-salt ATPS are presented to highlight their feasibility to operate as an attractive and versatile liquid-liquid extraction technology for the DSP of proteins and enzymes, thus facilitating the approach of new researchers to this technique. Thereby, single- and multi-stage extraction, several process integration methods, as well as large-scale extraction and purification of proteins regarding technical aspects, scale-up, recycling of process chemicals, and economic aspects are discussed. Springer-Verlag Berlin Heidelberg, 2015 Aqueous two-phase systems nationallicence Liquid-liquid extraction nationallicence Downstream processing nationallicence Aqueous two-phase extraction nationallicence Process integration nationallicence Large-scale extraction nationallicence Glyk Anna Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167, Hannover, Germany aut Scheper Thomas Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167, Hannover, Germany aut Beutel Sascha Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167, Hannover, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6599-6616 0175-7598 99:16<6599 2015 99 253 https://doi.org/10.1007/s00253-015-6779-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6779-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Glyk Anna Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167, Hannover, Germany aut NATIONALLICENCE 700 1- Scheper Thomas Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167, Hannover, Germany aut NATIONALLICENCE 700 1- Beutel Sascha Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167, Hannover, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6599-6616 0175-7598 99:16<6599 2015 99 253