Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6599-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6599-9 | ||
| 245 | 0 | 0 | |a Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 |h [Elektronische Daten] |c [Diego Salamanca, Rohan Karande, Andreas Schmid, Daniel Dobslaw] |
| 520 | 3 | |a Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5-C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5-C8) to their respective cyclic alcohols. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Cyclohexane |2 nationallicence | |
| 690 | 7 | |a CYP450 monooxygenase |2 nationallicence | |
| 690 | 7 | |a Cyclohexanol |2 nationallicence | |
| 690 | 7 | |a Cycloalkanes |2 nationallicence | |
| 690 | 7 | |a Biodegradation |2 nationallicence | |
| 700 | 1 | |a Salamanca |D Diego |u Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany |4 aut | |
| 700 | 1 | |a Karande |D Rohan |u Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany |4 aut | |
| 700 | 1 | |a Schmid |D Andreas |u Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany |4 aut | |
| 700 | 1 | |a Dobslaw |D Daniel |u Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/16(2015-08-01), 6889-6897 |x 0175-7598 |q 99:16<6889 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6599-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6599-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Salamanca |D Diego |u Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Karande |D Rohan |u Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schmid |D Andreas |u Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dobslaw |D Daniel |u Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/16(2015-08-01), 6889-6897 |x 0175-7598 |q 99:16<6889 |1 2015 |2 99 |o 253 | ||