caa a22 4500 605498385 CHVBK 20210128100546.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6599-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6599-9 Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 [Elektronische Daten] [Diego Salamanca, Rohan Karande, Andreas Schmid, Daniel Dobslaw] Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5-C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5-C8) to their respective cyclic alcohols. Springer-Verlag Berlin Heidelberg, 2015 Cyclohexane nationallicence CYP450 monooxygenase nationallicence Cyclohexanol nationallicence Cycloalkanes nationallicence Biodegradation nationallicence Salamanca Diego Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany aut Karande Rohan Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany aut Schmid Andreas Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany aut Dobslaw Daniel Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6889-6897 0175-7598 99:16<6889 2015 99 253 https://doi.org/10.1007/s00253-015-6599-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6599-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salamanca Diego Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany aut NATIONALLICENCE 700 1- Karande Rohan Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany aut NATIONALLICENCE 700 1- Schmid Andreas Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany aut NATIONALLICENCE 700 1- Dobslaw Daniel Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6889-6897 0175-7598 99:16<6889 2015 99 253