caa a22 4500 605498393 CHVBK 20210128100546.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6620-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6620-3 Glucose concentration alters dissolved oxygen levels in liquid cultures of Beauveria bassiana and affects formation and bioefficacy of blastospores [Elektronische Daten] [Gabriel Mascarin, Mark Jackson, Nilce Kobori, Robert Behle, Christopher Dunlap, Ítalo Delalibera Júnior] The filamentous fungus Beauveria bassiana is an economically important pathogen of numerous arthropod pests and is able to grow in submerged culture as filaments (mycelia) or as budding yeast-like blastospores. In this study, we evaluated the effect of dissolved oxygen and high glucose concentrations on blastospore production by submerged cultures of two isolates of B. bassiana, ESALQ1432 and GHA. Results showed that maintaining adequate dissolved oxygen levels coupled with high glucose concentrations enhanced blastospore yields by both isolates. High glucose concentrations increased the osmotic pressure of the media and coincided with higher dissolved oxygen levels and increased production of significantly smaller blastospores compared with blastospores produced in media with lower concentrations of glucose. The desiccation tolerance of blastospores dried to less than 2.6% moisture was not affected by the glucose concentration of the medium but was isolate dependent. Blastospores of isolate ESALQ1432 produced in media containing 140g glucoseL−1 showed greater virulence toward whitefly nymphs (Bemisia tabaci) as compared with blastospores produced in media containing 40g glucoseL−1. These results suggest a synergistic effect between glucose concentration and oxygen availability on changing morphology and enhancing the yield and efficacy of blastospores of B. bassiana, thereby facilitating the development of a cost-effective production method for this blastospore-based bioinsecticide. Springer-Verlag Berlin Heidelberg (outside the USA), 2015 Mycoinsecticides nationallicence Blastospores nationallicence Morphogenesis nationallicence Osmoadaptation nationallicence Beauveria bassiana nationallicence Desiccation tolerance nationallicence Aeration nationallicence Virulence nationallicence Bemisia tabaci nationallicence Mascarin Gabriel EMBRAPA Rice and Beans, Rodovia GO-462, km 12, Zona Rural, C.P. 179, 75375-000, Santo Antônio de Goiás, GO, Brazil aut Jackson Mark United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut Kobori Nilce United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut Behle Robert United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut Dunlap Christopher United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut Delalibera Júnior Ítalo Department of Entomology and Acarology, Escola Superior de Agricultura "Luiz de Queiroz”, University of São Paulo (ESALQ-USP), Av. Pádua Dias, 11, C.P. 9, 13418-900, Piracicaba, SP, Brazil aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6653-6665 0175-7598 99:16<6653 2015 99 253 https://doi.org/10.1007/s00253-015-6620-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6620-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mascarin Gabriel EMBRAPA Rice and Beans, Rodovia GO-462, km 12, Zona Rural, C.P. 179, 75375-000, Santo Antônio de Goiás, GO, Brazil aut NATIONALLICENCE 700 1- Jackson Mark United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Kobori Nilce United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Behle Robert United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Dunlap Christopher United States Department of Agriculture, Agriculture Research Service, Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, 1815 N. University St, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Delalibera Júnior Ítalo Department of Entomology and Acarology, Escola Superior de Agricultura "Luiz de Queiroz”, University of São Paulo (ESALQ-USP), Av. Pádua Dias, 11, C.P. 9, 13418-900, Piracicaba, SP, Brazil aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6653-6665 0175-7598 99:16<6653 2015 99 253