caa a22 4500 605498407 CHVBK 20210128100546.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6644-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6644-8 Fruit peels support higher yield and superior quality bacterial cellulose production [Elektronische Daten] [Jyoti Kumbhar, Jyutika Rajwade, Kishore Paknikar] Fruit peels, also known as rinds or skins, are wastes readily available in large quantities. Here, we have used pineapple (PA) and watermelon (WM) peels as substrates in the culture media (containing 5% sucrose and 0.7% ammonium sulfate) for production of bacterial cellulose (BC). The bacterial culture used in the study, Komagataeibacter hansenii produced BC under static conditions as a pellicle at the air-liquid interface in standard Hestrin and Schramm (HS) medium. The yield obtained was ~3.0g/100ml (on a wet weight basis). The cellulosic nature of the pellicle was confirmed by CO2, H2O, N2, and SO2 (CHNS) analysis and Fourier transform infrared (FT-IR) spectroscopy. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) of the pellicle revealed the presence of flat twisted ribbonlike fibrils (70-130nm wide). X-ray diffraction analysis proved its crystalline nature (matching cellulose I) with a crystallinity index of 67%. When K. hansenii was grown in PA and WM media, BC yields were threefolds or fourfolds higher than those obtained in HS medium. Interestingly, textural characterization tests (viz., SEM, crystallinity index, resilience, hardness, adhesiveness, cohesiveness, springiness, shear energy and stress, and energy required for puncturing the pellicle) proved that the quality of BC produced in PA and WM media was superior to the BC produced in HS medium. These findings demonstrate the utility of the newly designed media for getting higher yields and better quality of BC, which could make fermentative production of BC more attractive on a commercial scale. Springer-Verlag Berlin Heidelberg, 2015 Komagataeibacter hansenii MCM B-967 nationallicence Bacterial cellulose nationallicence Pineapple peels nationallicence Watermelon peels nationallicence Textural properties nationallicence Kumbhar Jyoti Centre for Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, 411 004, Pune, India aut Rajwade Jyutika Centre for Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, 411 004, Pune, India aut Paknikar Kishore Centre for Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, 411 004, Pune, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6677-6691 0175-7598 99:16<6677 2015 99 253 https://doi.org/10.1007/s00253-015-6644-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6644-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumbhar Jyoti Centre for Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, 411 004, Pune, India aut NATIONALLICENCE 700 1- Rajwade Jyutika Centre for Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, 411 004, Pune, India aut NATIONALLICENCE 700 1- Paknikar Kishore Centre for Nanobioscience, Agharkar Research Institute, G. G. Agarkar Road, 411 004, Pune, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6677-6691 0175-7598 99:16<6677 2015 99 253