The production of ω-hydroxy palmitic acid using fatty acid metabolism and cofactor optimization in Escherichia coli
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| 008 | 210128e20150801xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6630-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6630-1 | ||
| 245 | 0 | 4 | |a The production of ω-hydroxy palmitic acid using fatty acid metabolism and cofactor optimization in Escherichia coli |h [Elektronische Daten] |c [Changmin Sung, Eunok Jung, Kwon-Young Choi, Jin-hyung Bae, Minsuk Kim, Joonwon Kim, Eun-Jung Kim, Pyoung Kim, Byung-Gee Kim] |
| 520 | 3 | |a Hydroxylated fatty acids (HFAs) are used as important precursors for bulk and fine chemicals in the chemical industry. Here, to overproduce long-chain (C16-C18) fatty acids and hydroxy fatty acid, their biosynthetic pathways including thioesterase (Lreu_0335) from Lactobacillus reuteri DSM20016, β-hydroxyacyl-ACP dehydratase (fabZ) from Escherichia coli, and a P450 system (i.e., CYP153A from Marinobacter aquaeolei VT8 and camA/camB from Pseudomonas putida ATCC17453) were overexpressed. Acyl-CoA synthase (fadD) involved in fatty acid degradation by β-oxidation was also deleted in E. coli BW25113. The engineered E. coli FFA4 strain without the P450 system could produce 503.0mg/l of palmitic (C16) and 508.4mg/l of stearic (C18) acids, of which the amounts are ca. 1.6- and 2.3-fold higher than those of the wild type. On the other hand, the E. coli HFA4 strain including the P450 system for ω-hydroxylation could produce 211.7mg/l of ω-hydroxy palmitic acid, which was 42.1 ± 0.1% of the generated palmitic acid, indicating that the hydroxylation reaction was the rate-determining step for the HFA production. For the maximum production of ω-hydroxy palmitic acid, NADH, i.e., an essential cofactor for P450 reaction, was overproduced by the integration of NAD+-dependent formate dehydrogenase (FDH) from Candida boidinii into E. coli chromosome and the deletion of alcohol dehydrogenase (ADH). Finally, the NADH-level-optimized E. coli strain produced 610mg/l of ω-hydroxy palmitic acid (ω-HPA), which was almost a threefold increase in its yield compared to the same strain without NADH overproduction. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Free fatty acid |2 nationallicence | |
| 690 | 7 | |a Hydroxy fatty acid |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 690 | 7 | |a P450 monooxygenase |2 nationallicence | |
| 690 | 7 | |a Cofactor optimization |2 nationallicence | |
| 700 | 1 | |a Sung |D Changmin |u Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Jung |D Eunok |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Choi |D Kwon-Young |u Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, Gyeonggi-do, South Korea |4 aut | |
| 700 | 1 | |a Bae |D Jin-hyung |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Minsuk |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Joonwon |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Eun-Jung |u School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Pyoung |u Bio Control Research Institute, JBF, 495 Ipmyeon-ro, Ipmyeon, Gokseong-gun, Jeonnam, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Byung-Gee |u Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, South Korea |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/16(2015-08-01), 6667-6676 |x 0175-7598 |q 99:16<6667 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6630-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6630-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sung |D Changmin |u Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jung |D Eunok |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Choi |D Kwon-Young |u Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, Gyeonggi-do, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bae |D Jin-hyung |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Minsuk |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Joonwon |u School of Chemical and Biological Engineering, Institute of Bioengineering, Seoul National University, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Eun-Jung |u School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Pyoung |u Bio Control Research Institute, JBF, 495 Ipmyeon-ro, Ipmyeon, Gokseong-gun, Jeonnam, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Byung-Gee |u Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/16(2015-08-01), 6667-6676 |x 0175-7598 |q 99:16<6667 |1 2015 |2 99 |o 253 | ||