Design and application of a synthetic DNA standard for real-time PCR analysis of microbial communities in a biogas digester
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| 005 | 20210128100546.0 | ||
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| 008 | 210128e20150801xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6721-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6721-z | ||
| 245 | 0 | 0 | |a Design and application of a synthetic DNA standard for real-time PCR analysis of microbial communities in a biogas digester |h [Elektronische Daten] |c [T. May, M. Koch-Singenstreu, J. Ebling, R. Stantscheff, L. Müller, F. Jacobi, D. Polag, F. Keppler, H. König] |
| 520 | 3 | |a A synthetic DNA fragment containing primer binding sites for the quantification of ten different microbial groups was constructed and evaluated as a reliable enumeration standard for quantitative real-time PCR (qPCR) analyses. This approach has been exemplary verified for the quantification of several methanogenic orders and families in a series of samples drawn from a mesophilic biogas plant. Furthermore, the total amounts of bacteria as well as the number of sulfate-reducing and propionic acid bacteria as potential methanogenic interaction partners were successfully determined. The obtained results indicated a highly dynamic microbial community structure which was distinctly affected by the organic loading rate, the substrate selection, and the amount of free volatile fatty acids in the fermenter. Methanosarcinales was the most predominant methanogenic order during the 3months of observation despite fluctuating process conditions. During all trials, the modified quantification standard indicated a maximum of reproducibility and efficiency, enabling this method to open up a wide range of novel application options. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a qPCR |2 nationallicence | |
| 690 | 7 | |a Microbial diversity |2 nationallicence | |
| 690 | 7 | |a Methanogenic archaea |2 nationallicence | |
| 690 | 7 | |a Anaerobic digester |2 nationallicence | |
| 690 | 7 | |a Biogas plants |2 nationallicence | |
| 700 | 1 | |a May |D T. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | |
| 700 | 1 | |a Koch-Singenstreu |D M. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | |
| 700 | 1 | |a Ebling |D J. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | |
| 700 | 1 | |a Stantscheff |D R. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | |
| 700 | 1 | |a Müller |D L. |u Deutsches Biomasseforschungszentrum gemeinnützige GmbH (DBFZ), Torgauer Straße 116, 04347, Leipzig, Germany |4 aut | |
| 700 | 1 | |a Jacobi |D F. |u Deutsches Biomasseforschungszentrum gemeinnützige GmbH (DBFZ), Torgauer Straße 116, 04347, Leipzig, Germany |4 aut | |
| 700 | 1 | |a Polag |D D. |u Institute of Earthsciences, Ruprecht-Karls University Heidelberg, Im Neuenheimer Feld 234-236, 69120, Heidelberg, Germany |4 aut | |
| 700 | 1 | |a Keppler |D F. |u Institute of Earthsciences, Ruprecht-Karls University Heidelberg, Im Neuenheimer Feld 234-236, 69120, Heidelberg, Germany |4 aut | |
| 700 | 1 | |a König |D H. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/16(2015-08-01), 6855-6863 |x 0175-7598 |q 99:16<6855 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6721-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6721-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a May |D T. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Koch-Singenstreu |D M. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ebling |D J. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stantscheff |D R. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Müller |D L. |u Deutsches Biomasseforschungszentrum gemeinnützige GmbH (DBFZ), Torgauer Straße 116, 04347, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jacobi |D F. |u Deutsches Biomasseforschungszentrum gemeinnützige GmbH (DBFZ), Torgauer Straße 116, 04347, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Polag |D D. |u Institute of Earthsciences, Ruprecht-Karls University Heidelberg, Im Neuenheimer Feld 234-236, 69120, Heidelberg, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Keppler |D F. |u Institute of Earthsciences, Ruprecht-Karls University Heidelberg, Im Neuenheimer Feld 234-236, 69120, Heidelberg, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a König |D H. |u Institute of Microbiology and Wine Research, Johannes Gutenberg University Mainz, Johann-Joachim Becherweg 15, 55128, Mainz, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/16(2015-08-01), 6855-6863 |x 0175-7598 |q 99:16<6855 |1 2015 |2 99 |o 253 | ||