caa a22 4500 605498431 CHVBK 20210128100546.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00253-015-6694-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6694-y Utilization of secondary-treated wastewater for the production of freshwater microalgae [Elektronische Daten] [C. Gómez-Serrano, M. Morales-Amaral, F. Acién, R. Escudero, J. Fernández-Sevilla, E. Molina-Grima] In this work, we studied the potential use of secondary-treated wastewater as nutrient source in the production of freshwater microalgae strains. Experiments were performed indoors in a semicontinuous mode, at 0.3day−1, simulating outdoor conditions. We demonstrated that all the tested strains can be produced by using only secondary-treated wastewater as the nutrient source. The utilization of secondary-treated wastewater imposes nutrient-limiting conditions, with maximal biomass productivity dropping to 0.5gl−1day−1 and modifies the biochemical composition of the biomass by increasing the amount of lipids and carbohydrates while reducing the biomass protein content. We measured fatty acid content and productivity of up to 25%d.wt. and 110mgl−1day−1, respectively. We demonstrated that all the tested strains were capable of completely removing the nitrogen and phosphorus contained in the secondary-treated wastewater, and while the use of this effluent reduced the cells' photosynthetic efficiency, the nitrogen and phosphorus coefficient yield increased. Muriellopsis sp. and S. subpicatus were selected as the most promising strains for outdoor production using secondary-treated wastewater as the culture medium; this was not only because of their high productivity but also their photosynthetic efficiency, of up to 2.5%, along with nutrient coefficient yields of up to 96gbiomassgN −1 and 166gbiomassgP −1. Coupling microalgae production processes to tertiary treatment in wastewater treatment plants make it possible to recover nutrients contained in the water and to produce valuable biomass, especially where nutrient removal is required prior to wastewater discharge. Springer-Verlag Berlin Heidelberg, 2015 Microalgae nationallicence Wastewater nationallicence Nutrient limitation nationallicence Productivity nationallicence Biochemical composition nationallicence Light efficiency nationallicence Gómez-Serrano C. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut Morales-Amaral M. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut Acién F. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut Escudero R. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut Fernández-Sevilla J. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut Molina-Grima E. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6931-6944 0175-7598 99:16<6931 2015 99 253 https://doi.org/10.1007/s00253-015-6694-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6694-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gómez-Serrano C. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut NATIONALLICENCE 700 1- Morales-Amaral M. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut NATIONALLICENCE 700 1- Acién F. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut NATIONALLICENCE 700 1- Escudero R. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut NATIONALLICENCE 700 1- Fernández-Sevilla J. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut NATIONALLICENCE 700 1- Molina-Grima E. Department of Chemical Engineering, University of Almería, Carretera Sacramento s/n, E04120, Almería, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/16(2015-08-01), 6931-6944 0175-7598 99:16<6931 2015 99 253