A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6831-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6831-7 | ||
| 245 | 0 | 2 | |a A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium |h [Elektronische Daten] |c [Véronique Wuyts, Wesley Mattheus, Nancy Roosens, Kathleen Marchal, Sophie Bertrand, Sigrid De Keersmaecker] |
| 520 | 3 | |a Subtyping below the serovar level is essential for surveillance and outbreak detection and investigation of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) and its monophasic variant 1,4,[5],12:i:- (S. 1,4,[5],12:i:-), frequent causes of foodborne infections. In an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation-PCR (MOL-PCR) assay was developed which combines different types of molecular markers in a high-throughput microsphere suspension array. The 52 molecular markers include prophage genes, amplified fragment length polymorphism (AFLP) elements, Salmonella genomic island 1 (SGI1), allantoinase gene allB, MLVA locus STTR10, antibiotic resistance genes, single nucleotide polymorphisms (SNPs) and phase 2 flagellar gene fljB. The in vitro stability of these markers was confirmed in a serial passage experiment. The validation of the MOL-PCR assay for subtyping of S. Typhimurium and S. 1,4,[5],12:i:- on 519 isolates shows that the method is rapid, reproducible, flexible, accessible, easy to use and relatively inexpensive. Additionally, a 100% typeability and a discriminatory power equivalent to that of phage typing were observed, and epidemiological concordance was assessed on isolates of 2 different outbreaks. Furthermore, a data analysis method is provided so that the MOL-PCR assay allows for objective, computerised data analysis and data interpretation of which the results can be easily exchanged between different laboratories in an international surveillance network. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a MOL-PCR |2 nationallicence | |
| 690 | 7 | |a Salmonella Typhimurium |2 nationallicence | |
| 690 | 7 | |a Subtyping |2 nationallicence | |
| 690 | 7 | |a Luminex |2 nationallicence | |
| 690 | 7 | |a Microsphere suspension array |2 nationallicence | |
| 700 | 1 | |a Wuyts |D Véronique |u Department of Microbial and Molecular Systems, KU Leuven, Kasteelpark Arenberg 20 bus 2460, 3001, Leuven, Belgium |4 aut | |
| 700 | 1 | |a Mattheus |D Wesley |u National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | |
| 700 | 1 | |a Roosens |D Nancy |u Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | |
| 700 | 1 | |a Marchal |D Kathleen |u Department of Microbial and Molecular Systems, KU Leuven, Kasteelpark Arenberg 20 bus 2460, 3001, Leuven, Belgium |4 aut | |
| 700 | 1 | |a Bertrand |D Sophie |u National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | |
| 700 | 1 | |a De Keersmaecker |D Sigrid |u Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 8137-8149 |x 0175-7598 |q 99:19<8137 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6831-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6831-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wuyts |D Véronique |u Department of Microbial and Molecular Systems, KU Leuven, Kasteelpark Arenberg 20 bus 2460, 3001, Leuven, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mattheus |D Wesley |u National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Roosens |D Nancy |u Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marchal |D Kathleen |u Department of Microbial and Molecular Systems, KU Leuven, Kasteelpark Arenberg 20 bus 2460, 3001, Leuven, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bertrand |D Sophie |u National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a De Keersmaecker |D Sigrid |u Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health (WIV-ISP), Juliette Wytmanstraat 14, 1050, Brussels, Belgium |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 8137-8149 |x 0175-7598 |q 99:19<8137 |1 2015 |2 99 |o 253 | ||