caa a22 4500 605498547 CHVBK 20210128100546.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6733-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6733-8 Single cell oil production on molasses by Yarrowia lipolytica strains overexpressing DGA2 in multicopy [Elektronische Daten] [Peter Gajdoš, Jean-Marc Nicaud, Tristan Rossignol, Milan Čertík] Yarrowia lipolytica is a promising platform for single cell oil production. It is well-known for its metabolism oriented toward utilization of hydrophobic substrates and accumulation of storage lipids. Multiple copies of DGA2 under constitutive promoter were introduced into the Q4 strain, a quadruple mutant deleted for the four acyltransferases (Δdga1, Δdga2, Δlro1, and Δare1) to improve lipid accumulation. The Q4-DGA2 x3 strain contains three copies of DGA2. Further increase in accumulation was accomplished by blocking the β-oxidation pathway through MFE1 gene deletion yielding Q4-Δmfe DGA2 x3. In order to use molasses as a substrate for single cell oil production, sucrose utilization was established by expressing the Saccharomyces cerevisiae SUC2 gene yielding Q4-SUC2 DGA2 x3 and Q4-Δmfe SUC2 DGA2 x3. During cultivation on sucrose medium with a carbon to nitrogen ratio of 80, both strains accumulated more than 40% of lipids, which was a 2-fold increase in lipid storage. Q4-Δmfe SUC2 DGA2 x3 accumulated more lipids than Q4-SUC2 DGA2 x3 (49 vs. 43%) but yielded less biomass (13.7 vs. 15g/L). When grown on 8% (v/v) molasses, both strains accumulated more than 30% of lipids after 3days, while biomass yield was higher in Q4-SUC2 DGA2 x3 (16.4 vs. 14.4g/L). Further addition of molasses at 72h resulted in higher biomass yield, 26.6g/L for Q4-SUC2 DGA2 x3, without modification of lipid content. This work presents genetically modified strains of Y. lipolytica as suitable tools for direct conversion of industrial molasses into value added products based on single cell oils. Springer-Verlag Berlin Heidelberg, 2015 Yarrowia lipolytica nationallicence Oleaginous yeast nationallicence Lipid metabolism nationallicence Molasses nationallicence Biodiesel nationallicence DGAT nationallicence Gajdoš Peter Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut Nicaud Jean-Marc INRA, UMR1319 Micalis, F-78352, Jouy-en-Josas, France aut Rossignol Tristan INRA, UMR1319 Micalis, F-78352, Jouy-en-Josas, France aut Čertík Milan Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8065-8074 0175-7598 99:19<8065 2015 99 253 https://doi.org/10.1007/s00253-015-6733-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6733-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gajdoš Peter Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Nicaud Jean-Marc INRA, UMR1319 Micalis, F-78352, Jouy-en-Josas, France aut NATIONALLICENCE 700 1- Rossignol Tristan INRA, UMR1319 Micalis, F-78352, Jouy-en-Josas, France aut NATIONALLICENCE 700 1- Čertík Milan Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8065-8074 0175-7598 99:19<8065 2015 99 253