caa a22 4500 605498555 CHVBK 20210128100547.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6703-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6703-1 Secreted xylanase XynA mediates utilization of xylan as sole carbon source in Candida utilis [Elektronische Daten] [Maya Kunigo, Christoph Buerth, Joachim Ernst] The fodder yeast Candida utilis is able to use xylose mono- and oligomers as sources of carbon but not the abundant polymer xylan. C. utilis transformants producing the Penicillium simplicissimum xylanase XynA were constructed using expression vectors encoding fusions of the Saccharomyces cerevisiae Mfα1 pre-pro secretion leader to XynA. The Mfα1-XynA fusion was efficiently processed in transformants and XynA was secreted almost quantitatively into the culture medium. Secreted XynA was enzymatically active and allowed transformants to grow on xylan as the sole carbon source. Addition of a second expression unit for the heterologous green fluorescent protein (GFP) generated C. utilis transformants, which showed intracellular GFP fluorescence during growth on xylan. The results suggest that xylanase-producing C. utilis is suited as a cost-effective host organism for heterologous protein production and for other biotechnical applications. Springer-Verlag Berlin Heidelberg, 2015 Candida utilis nationallicence Yeast nationallicence Xylan nationallicence Xylanase nationallicence Xylose nationallicence Heterologous expression nationallicence Kunigo Maya Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Universitätsstrasse 1/26.12.01, 40225, Düsseldorf, Germany aut Buerth Christoph Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Universitätsstrasse 1/26.12.01, 40225, Düsseldorf, Germany aut Ernst Joachim Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Universitätsstrasse 1/26.12.01, 40225, Düsseldorf, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8055-8064 0175-7598 99:19<8055 2015 99 253 https://doi.org/10.1007/s00253-015-6703-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6703-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kunigo Maya Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Universitätsstrasse 1/26.12.01, 40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Buerth Christoph Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Universitätsstrasse 1/26.12.01, 40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Ernst Joachim Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Universitätsstrasse 1/26.12.01, 40225, Düsseldorf, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8055-8064 0175-7598 99:19<8055 2015 99 253