caa a22 4500 605498628 CHVBK 20210128100547.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6670-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6670-6 The role of paraffin oil on the interaction between denitrifying anaerobic methane oxidation and Anammox processes [Elektronische Daten] [Liang Fu, Zhao-Wei Ding, Jing Ding, Fang Zhang, Raymond Zeng] Methane is sparingly soluble in water, resulting in a slow reaction rate in the denitrifying anaerobic methane oxidation (DAMO) process. The slow rate limits the feasibility of research to examine the interaction between the DAMO and the anaerobic ammonium oxidation (Anammox) process. In this study, optimized 5% (v/v) paraffin oil was added as a second liquid phase to improve methane solubility in a reactor containing DAMO and Anammox microbes. After just addition, methane solubility was found to increase by 25% and DAMO activity was enhanced. After a 100-day cultivation, the paraffin reactor showed almost two times higher consumption rates of NO3 − (0.2268mmol/day) and NH4 + (0.1403mmol/day), compared to the control reactor without paraffin oil. The microbes tended to distribute in the oil-water interface. The quantitative (q) PCR result showed the abundance of gene copies of DAMO archaea, DAMO bacteria, and Anammox bacteria in the paraffin reactor were higher than those in the control reactor after 1month. Fluorescence in situ hybridization revealed that the percentages of the three microbes were 55.5 and 77.6% in the control and paraffin reactors after 100days, respectively. A simple model of mass balance was developed to describe the interactions between DAMO and Anammox microbes and validate the activity results. A mechanism was proposed to describe the possible way that paraffin oil enhanced DAMO activity. It is quite clear that paraffin oil enhances not only DAMO activity but also Anammox activity via the interaction between them; both NO3 − and NH4 + consumption rates were about two times those of the control. Springer-Verlag Berlin Heidelberg, 2015 Paraffin oil nationallicence Denitrifying anaerobic methane oxidation nationallicence Anammox nationallicence Interspecies interaction nationallicence Gas transfer nationallicence Fu Liang CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut Ding Zhao-Wei CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut Ding Jing CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut Zhang Fang CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut Zeng Raymond CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 7925-7936 0175-7598 99:19<7925 2015 99 253 https://doi.org/10.1007/s00253-015-6670-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6670-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fu Liang CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut NATIONALLICENCE 700 1- Ding Zhao-Wei CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut NATIONALLICENCE 700 1- Ding Jing CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut NATIONALLICENCE 700 1- Zhang Fang CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut NATIONALLICENCE 700 1- Zeng Raymond CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 7925-7936 0175-7598 99:19<7925 2015 99 253