caa a22 4500 605498644 CHVBK 20210128100547.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6674-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6674-2 The effect of tryptophol on the bacteriophage infection in high-temperature environment [Elektronische Daten] [Min Jin, Chenxi Xu, Xiaobo Zhang] Small metabolites can participate in the virus-host interactions in eukaryotes. However, little is known about roles of metabolites in the interactions between bacteria and bacteriophages. In this study, the metabolomic profilings of bacteriophage GVE2-infected and virus-free Geobacillus sp. E263, a thermophilic bacterium isolated from a deep-sea hydrothermal vent, were characterized. The results showed that metabolites tryptophol, adenine, and hydroxybenzylalcohol were significantly elevated in Geobacillus sp. E263 in response to the GVE2 infection. Furthermore, our data indicated that tryptophol was involved in the bacteriophage infection. Tryptophol could inhibit the infection/replication of GVE2 by interacting with the host's Clp protease. Therefore, our study revealed novel aspects of metabolites during the bacteriophage infection in high-temperature environment. Springer-Verlag Berlin Heidelberg, 2015 Bacteriophage nationallicence Thermophiles nationallicence Tryptophol nationallicence Metabolic interaction nationallicence Jin Min Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Xu Chenxi Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Zhang Xiaobo Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8101-8111 0175-7598 99:19<8101 2015 99 253 https://doi.org/10.1007/s00253-015-6674-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6674-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Min Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Xu Chenxi Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Xiaobo Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and College of Life Sciences, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8101-8111 0175-7598 99:19<8101 2015 99 253