caa a22 4500 605498652 CHVBK 20210128100547.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6726-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6726-7 Cells of Escherichia coli are protected against severe chemical stress by co-habiting cell aggregates formed by Pseudomonas aeruginosa [Elektronische Daten] [Nina Jagmann, Sebastian Henke, Bodo Philipp] Bacterial cells within biofilms and cell aggregates show increased resistance against chemical stress compared with suspended cells. It is not known whether bacteria that co-habit biofilms formed by other bacteria also acquire such resistance. This scenario was investigated in a proof-of-principle experiment with Pseudomonas aeruginosa strain PAO1 as cell aggregate-forming bacterium and Escherichia coli strain MG1655 as potential co-habiting bacterium equipped with an inducible bioluminescence system. Cell aggregation of strain PAO1 can be induced by the toxic detergent sodium dodecyl sulfate (SDS). In single cultures of strain MG1655, bioluminescence was inhibited by the protonophor carbonylcyanide-m-chlorophenylhydrazone (CCCP) but the cells were still viable. By applying CCCP and SDS together, cells of strain MG1655 lost their bioluminescence and viability indicating the importance of energy-dependent resistance mechanisms against SDS. In co-suspensions with strain PAO1, bioluminescence of strain MG1655 was sustained in the presence of SDS and CCCP. Image analysis showed that bioluminescent cells were located in cell aggregates formed by strain PAO1. Thus, cells of strain MG1655 that co-habited cell aggregates formed by strain PAO1 were protected against a severe chemical stress that was lethal to them in single cultures. Co-habiting could lead to increased survival of pathogens in clinical settings and could be employed in biotechnological applications involving toxic milieus. Springer-Verlag Berlin Heidelberg, 2015 Mixed-species biofilm nationallicence Cell aggregation nationallicence Chemical stress resistance nationallicence Pseudomonas aeruginosa nationallicence Escherichia coli nationallicence Bioluminescence nationallicence Jagmann Nina Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität (WWU) Münster, Corrensstr. 3, 48149, Münster, Germany aut Henke Sebastian Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität (WWU) Münster, Corrensstr. 3, 48149, Münster, Germany aut Philipp Bodo Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität (WWU) Münster, Corrensstr. 3, 48149, Münster, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8285-8294 0175-7598 99:19<8285 2015 99 253 https://doi.org/10.1007/s00253-015-6726-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6726-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jagmann Nina Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität (WWU) Münster, Corrensstr. 3, 48149, Münster, Germany aut NATIONALLICENCE 700 1- Henke Sebastian Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität (WWU) Münster, Corrensstr. 3, 48149, Münster, Germany aut NATIONALLICENCE 700 1- Philipp Bodo Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität (WWU) Münster, Corrensstr. 3, 48149, Münster, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8285-8294 0175-7598 99:19<8285 2015 99 253