caa a22 4500 605498695 CHVBK 20210128100547.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6690-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6690-2 Characterization of a novel β-cypermethrin-degrading Aspergillus niger YAT strain and the biochemical degradation pathway of β-cypermethrin [Elektronische Daten] [Weiqin Deng, Derong Lin, Kai Yao, Huaiyu Yuan, Zhilong Wang, Jianlong Li, Likou Zou, Xinfeng Han, Kang Zhou, Li He, Xinjie Hu, Shuliang Liu] Aspergillus niger YAT strain was obtained from Chinese brick tea (Collection number: CGMCC 10,568) and identified on the basis of morphological characteristics and internal transcribed spacer (ITS) sequence. The strain could degrade 54.83% of β-cypermethrin (β-CY; 50mgL−1) in 7days and 100% of 3-phenoxybenzoic acid (3-PBA; 100mgL−1) in 22h. The half-lives of β-CY and 3-PBA range from 3.573 to 11.748 days and from 5.635 to 12.160h, respectively. The degradation of β-CY and 3-PBA was further described using first-order kinetic models. The pathway and mechanism of β-CY degraded by YAT were investigated by analyzing the degraded metabolites through high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Relevant enzymatic activities and substrate utilization were also investigated. β-CY degradation products were analyzed. Results indicated that YAT strain transformed β-CY into 3-PBA. 3-PBA was then gradually transformed into permethric acid, protocatechuic acid, 3-hydroxy-5-phenoxy benzoic acid, gallic acid, and phenol gradually. The YAT strain can also effectively degrade these metabolites. The results indicated that YAT strain has potential applications in bioremediation of pyrethroid insecticide (PI)-contaminated environments and fermented food. Springer-Verlag Berlin Heidelberg, 2015 β-cypermethrin nationallicence 3-phenoxybenzoic acid nationallicence Biodegradation nationallicence Degradation pathway nationallicence Aspergillus niger YAT strain nationallicence Deng Weiqin College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Lin Derong College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Yao Kai College of Light Industry and Food, Sichuan University, 610065, Chengdu, Sichuan, People's Republic of China aut Yuan Huaiyu College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Wang Zhilong College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Li Jianlong College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Zou Likou The Laboratory of Microbiology, Dujiangyan Campus, Sichuan Agricultural University, 611830, Dujiangyan, Sichuan, People's Republic of China aut Han Xinfeng College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Zhou Kang College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut He Li College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Hu Xinjie College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Liu Shuliang College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8187-8198 0175-7598 99:19<8187 2015 99 253 https://doi.org/10.1007/s00253-015-6690-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6690-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Deng Weiqin College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Lin Derong College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Yao Kai College of Light Industry and Food, Sichuan University, 610065, Chengdu, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Huaiyu College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhilong College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Li Jianlong College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Zou Likou The Laboratory of Microbiology, Dujiangyan Campus, Sichuan Agricultural University, 611830, Dujiangyan, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Han Xinfeng College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Kang College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- He Li College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Hu Xinjie College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 700 1- Liu Shuliang College of Food Science, Sichuan Agricultural University, 625014, Ya'an, Sichuan, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8187-8198 0175-7598 99:19<8187 2015 99 253