Biosynthesis of 2-deoxysugars using whole-cell catalyst expressing 2-deoxy- d -ribose 5-phosphate aldolase
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| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6740-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6740-9 | ||
| 245 | 0 | 0 | |a Biosynthesis of 2-deoxysugars using whole-cell catalyst expressing 2-deoxy- d -ribose 5-phosphate aldolase |h [Elektronische Daten] |c [Jitao Li, Jiangang Yang, Yan Men, Yan Zeng, Yueming Zhu, Caixia Dong, Yuanxia Sun, Yanhe Ma] |
| 520 | 3 | |a 2-Deoxy-d-ribose 5-phosphate aldolase (DERA) accepts a wide variety of aldehydes and is used in de novo synthesis of 2-deoxysugars, which have important applications in drug manufacturing. However, DERA has low preference for non-phosphorylated substrates. In this study, DERA from Klebsiella pneumoniae (KDERA) was mutated to increase its enzyme activity and substrate tolerance towards non-phosphorylated polyhydroxy aldehyde. Mutant KDERAK12 (S238D/F200I/ΔY259) showed a 3.15-fold improvement in enzyme activity and a 1.54-fold increase in substrate tolerance towards d-glyceraldehyde compared with the wild type. Furthermore, a whole-cell transformation strategy using resting cells of the BL21(pKDERA12) strain, containing the expressed plasmid pKDERA12, resulted in increase in 2-deoxy-d-ribose yield from 0.41mol/mol d-glyceraldehyde to 0.81mol/mol d-glyceraldehyde and higher substrate tolerance from 0.5 to 3M compared to in vitro assays. With further optimization of the transformation process, the BL21(pKDERA12) strain produced 2.14M (287.06g/L) 2-deoxy-d-robose (DR), with a yield of 0.71mol/mol d-glyceraldehyde and average productivity of 0.13mol/L·h (17.94g/L·h). These results demonstrate the potential for large-scale production of 2-deoxy-d-ribose using the BL21(pKDERA12) strain. Furthermore, the BL21(pKDERA12) strain also exhibited the ability to efficiently produce 2-deoxy-d-altrose from d-erythrose, as well as 2-deoxy-l-xylose and 2-deoxy-l-ribose from l-glyceraldehyde. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a 2-Deoxy- d -ribose 5-phosphate aldolase (DERA) |2 nationallicence | |
| 690 | 7 | |a Deoxysugars |2 nationallicence | |
| 690 | 7 | |a Whole-cell transformation |2 nationallicence | |
| 690 | 7 | |a Directed mutagenesis |2 nationallicence | |
| 700 | 1 | |a Li |D Jitao |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Yang |D Jiangang |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Men |D Yan |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Zeng |D Yan |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Zhu |D Yueming |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Dong |D Caixia |u Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, 300070, Tianjin, China |4 aut | |
| 700 | 1 | |a Sun |D Yuanxia |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Ma |D Yanhe |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 7963-7972 |x 0175-7598 |q 99:19<7963 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6740-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6740-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Jitao |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Jiangang |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Men |D Yan |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zeng |D Yan |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhu |D Yueming |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dong |D Caixia |u Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, 300070, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Yuanxia |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ma |D Yanhe |u National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 7963-7972 |x 0175-7598 |q 99:19<7963 |1 2015 |2 99 |o 253 | ||