Systematic analysis of intracellular mechanisms of propanol production in the engineered Thermobifida fusca B6 strain
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| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6850-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6850-4 | ||
| 245 | 0 | 0 | |a Systematic analysis of intracellular mechanisms of propanol production in the engineered Thermobifida fusca B6 strain |h [Elektronische Daten] |c [Yu Deng, Adam Fisher, Stephen Fong] |
| 520 | 3 | |a Thermobifida fusca is a moderately thermophilic actinobacterium naturally capable of utilizing lignocellulosic biomass. The B6 strain of T. fusca was previously engineered to produce 1-propanol directly on lignocellulosic biomass by expressing a bifunctional butyraldehyde/alcohol dehydrogenase (adhE2). To characterize the intracellular mechanisms related to the accumulation of 1-propanol, the engineered B6 and wild-type (WT) strains were systematically compared by analysis of the transcriptome and intracellular metabolome during exponential growth on glucose, cellobiose, and Avicel. Of the 18 known cellulases in T. fusca, 10 cellulase genes were transcriptionally expressed on all three substrates along with three hemicellulases. Transcriptomic analysis of cellodextrin and cellulose transport revealed that Tfu_0936 (multiple sugar transport system permease) was the key enzyme regulating the uptake of sugars in T. fusca. For both WT and B6 strains, it was found that growth in oxygen-limited conditions resulted in a blocked tricarboxylic acid (TCA) cycle caused by repressed expression of Tfu_1925 (aconitate hydratase). Further, the transcriptome suggested a pathway for synthesizing succinyl-CoA: oxaloacetate to malate (by malate dehydrogenase), malate to fumarate (by fumarate hydratase), and fumarate to succinate (by succinate dehydrogenase/fumarate reductase) which was ultimately converted to succinyl-CoA by succinyl-CoA synthetase. Both the transcriptome and the intracellular metabolome confirmed that 1-propanol was produced through succinyl-CoA, L-methylmalonyl-CoA, D-methylmalonyl-CoA, and propionyl-CoA in the B6 strain. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Actinobacterium |2 nationallicence | |
| 690 | 7 | |a 1-Propanol |2 nationallicence | |
| 690 | 7 | |a Transcriptomics |2 nationallicence | |
| 690 | 7 | |a Synthetic pathway |2 nationallicence | |
| 700 | 1 | |a Deng |D Yu |u National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China |4 aut | |
| 700 | 1 | |a Fisher |D Adam |u Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 W. Main Street, 23284, Richmond, VA, USA |4 aut | |
| 700 | 1 | |a Fong |D Stephen |u Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 W. Main Street, 23284, Richmond, VA, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 8089-8100 |x 0175-7598 |q 99:19<8089 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6850-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6850-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Deng |D Yu |u National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fisher |D Adam |u Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 W. Main Street, 23284, Richmond, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fong |D Stephen |u Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 W. Main Street, 23284, Richmond, VA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/19(2015-10-01), 8089-8100 |x 0175-7598 |q 99:19<8089 |1 2015 |2 99 |o 253 | ||