caa a22 4500 605498822 CHVBK 20210128100548.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6845-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6845-1 Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes [Elektronische Daten] [Shinji Hama, Chiaki Ogino, Akihiko Kondo] Phospholipids (PLs) containing specific polar head groups and fatty acids, artificially synthesized from a complex mixture of natural PLs, have considerable industrial applications. The biocatalytic approaches to synthesizing structured PLs are of great interest because the enzymes used show high selectivity and performance under mild conditions, leading to the generation of products that cannot easily be obtained by chemical catalysis. Although the limited supply of phospholipases (e.g., phospholipase D) has thus far been an obstacle to the widespread use of enzymatic processing, recent advances in enzyme preparation have opened up various applications for PL modification. In this review, attempts to increase the productivity and utility of microbial phospholipases and lipases are presented. We also summarize recent developments in enzyme-catalyzed modification of PLs, focusing particularly on the relevant reactions, bioreactor design, and novel proof-of-concept experiments. Springer-Verlag Berlin Heidelberg, 2015 Bioreactor nationallicence Enzyme production nationallicence Lecithin nationallicence Phospholipases nationallicence Phospholipid modification nationallicence Hama Shinji Research & Development Laboratory, Bio-energy Corporation, 2-9-7 Minaminanamatsu, 660-0053, Amagasaki, Japan aut Ogino Chiaki Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan aut Kondo Akihiko Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 7879-7891 0175-7598 99:19<7879 2015 99 253 https://doi.org/10.1007/s00253-015-6845-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6845-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hama Shinji Research & Development Laboratory, Bio-energy Corporation, 2-9-7 Minaminanamatsu, 660-0053, Amagasaki, Japan aut NATIONALLICENCE 700 1- Ogino Chiaki Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan aut NATIONALLICENCE 700 1- Kondo Akihiko Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 7879-7891 0175-7598 99:19<7879 2015 99 253