caa a22 4500 605498903 CHVBK 20210128100548.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00253-015-6832-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6832-6 Adaptation of acidogenic sludge to increasing glycerol concentrations for biohydrogen production [Elektronische Daten] [E. Tapia-Venegas, L. Cabrol, B. Brandhoff, J. Hamelin, E. Trably, JP Steyer, G. Ruiz-Filippi] Hydrogen is a promising alternative as an energetic carrier and its production by dark fermentation from wastewater has been recently proposed, with special attention to crude glycerol as potential substrate. In this study, two different feeding strategies were evaluated for replacing the glucose substrate by glycerol substrate: a one-step strategy (glucose was replaced abruptly by glycerol) and a step-by-step strategy (progressive decrease of glucose concentration and increase of glycerol concentration from 0 to 5gL−1), in a continuous stirred tank reactor (12h of hydraulic retention time (HRT), pH 5.5, 35°C). While the one-step strategy led to biomass washout and unsuccessful H2 production, the step-by-step strategy was efficient for biomass adaptation, reaching acceptable hydrogen yields (0.4 ± 0.1 molH2 mol−1 glycerol consumed) around 33% of the theoretical yield independently of the glycerol concentration. Microbial community structure was investigated by single-strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) fingerprinting techniques, targeting either the total community (16S ribosomal RNA (rRNA) gene) or the functional Clostridium population involved in H2 production (hydA gene), as well as by 454 pyrosequencing of the total community. Multivariate analysis of fingerprinting and pyrosequencing results revealed the influence of the feeding strategy on the bacterial community structure and suggested the progressive structural adaptation of the community to increasing glycerol concentrations, through the emergence and selection of specific species, highly correlated to environmental parameters. Particularly, this work highlighted an interesting shift of dominant community members (putatively responsible of hydrogen production in the continuous stirred tank reactor (CSTR)) according to the gradient of glycerol proportion in the feed, from the family Veillonellaceae to the genera Prevotella and Clostridium sp., putatively responsible of hydrogen production in the CSTR. Springer-Verlag Berlin Heidelberg, 2015 Acidogenic sludge adaptation nationallicence Biohydrogen nationallicence Glycerol nationallicence Dark fermentation nationallicence Community structure nationallicence Pyrosequencing nationallicence Tapia-Venegas E. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut Cabrol L. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut Brandhoff B. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut Hamelin J. INRA, UR 0050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100, Narbonne, France aut Trably E. INRA, UR 0050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100, Narbonne, France aut Steyer JP INRA, UR 0050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100, Narbonne, France aut Ruiz-Filippi G. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8295-8308 0175-7598 99:19<8295 2015 99 253 https://doi.org/10.1007/s00253-015-6832-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6832-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tapia-Venegas E. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 700 1- Cabrol L. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 700 1- Brandhoff B. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 700 1- Hamelin J. INRA, UR 0050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100, Narbonne, France aut NATIONALLICENCE 700 1- Trably E. INRA, UR 0050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100, Narbonne, France aut NATIONALLICENCE 700 1- Steyer JP INRA, UR 0050, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100, Narbonne, France aut NATIONALLICENCE 700 1- Ruiz-Filippi G. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/19(2015-10-01), 8295-8308 0175-7598 99:19<8295 2015 99 253