caa a22 4500 605498954 CHVBK 20210128100548.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-015-6427-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6427-2 Feasibility of mini-sequencing schemes based on nucleotide polymorphisms for microbial identification and population analyses [Elektronische Daten] [Ricardo Araujo, Nadia Eusebio, Rita Caramalho] Practical schemes based on single nucleotide polymorphisms (SNP) have been proposed as alternatives to simplify and replace the molecular methodologies based on the extensive sequencing analysis of genes. SNaPshot mini-sequencing has been progressively experienced during the last decade and represents a fast and robust strategy to analyze critical polymorphisms. Such assays have been proposed to characterize some bacteria and microbial eukaryotes, and its feasibility was now reviewed in the present manuscript. The mini-sequencing schemes showed high discriminatory power and competence for identification of microorganisms, but some specificity errors were still found, particularly for species of the Burkholderia cepacia complex and mycobacteria. SNP assays designed for other goals, e.g., comparison of strains, detection of serotypes, virulence, epidemic, and phylogenetic-related subgroups of isolates, can be very useful by facilitating the investigation of large collections of isolates. The next-generation of SNP assays might consider the inclusion of large number of markers to fully characterize microbial taxonomy and strains; nevertheless, these new technologies are still prone to errors and can largely benefit from integration with well-established mini-sequencing assays. Newly proposed molecular tools should be systematically tested in collections of isolates with high indexes of diversity and guarantee interlaboratorial validation. Springer-Verlag Berlin Heidelberg, 2015 Genetic diversity nationallicence Multiplex primer extension assay nationallicence SNaPshot nationallicence SNP nationallicence Taxonomy nationallicence Araujo Ricardo IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465, Porto, Portugal aut Eusebio Nadia IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465, Porto, Portugal aut Caramalho Rita IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465, Porto, Portugal aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2513-2521 0175-7598 99:6<2513 2015 99 253 https://doi.org/10.1007/s00253-015-6427-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6427-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Araujo Ricardo IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465, Porto, Portugal aut NATIONALLICENCE 700 1- Eusebio Nadia IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465, Porto, Portugal aut NATIONALLICENCE 700 1- Caramalho Rita IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465, Porto, Portugal aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2513-2521 0175-7598 99:6<2513 2015 99 253