A novel chemoreceptor MCP2983 from Comamonas testosteroni specifically binds to cis -aconitate and triggers chemotaxis towards diverse organic compounds
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6216-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6216-3 | ||
| 245 | 0 | 2 | |a A novel chemoreceptor MCP2983 from Comamonas testosteroni specifically binds to cis -aconitate and triggers chemotaxis towards diverse organic compounds |h [Elektronische Daten] |c [Bin Ni, Zhou Huang, Yu-Fan Wu, Zheng Fan, Cheng-Ying Jiang, Shuang-Jiang Liu] |
| 520 | 3 | |a Comamonas testosteroni CNB-1 behaves chemotactically toward a wide range of organic compounds, and 19 methyl-accepting chemotaxis proteins (MCPs) were annotated from the genome of strain CNB-2, a plasmid-curing derivative from strain CNB-1. The MCP-free mutant CNB-1Δ20 completely lost its chemotactic responses. In this study, we found that a chemoreceptor, namely MCP2983, restored chemotactic responses toward nine carboxylic acids and ten aromatic compounds to CNB-1Δ20. Isothermal titration calorimetry analysis indicated that the ligand-binding domain (LBD) of MCP2983 specifically binds to cis-aconitate but not other tested compounds. Deletion of the LBD of MCP2983 impaired chemotactic responses toward cis-aconitate as well as other tested compounds, indicating that the LBD of MCP2983 was essential for triggering chemotactic responses. Five amino acid residues (M81, S156, E157, I158, and L159) that are located at a putative ligand-binding pocket were identified to be involved in binding to cis-aconitate. So far, the MCP2983 represents the sole biochemically identified chemoreceptor that specifically binds to cis-aconitate and is able to trigger chemotaxis towards diverse organic compounds. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Methyl-accepting chemotaxis proteins (MCPs) |2 nationallicence | |
| 690 | 7 | |a cis -Aconitate |2 nationallicence | |
| 690 | 7 | |a Chemoreceptor |2 nationallicence | |
| 690 | 7 | |a Tricarboxylic acid (TCA) cycle |2 nationallicence | |
| 690 | 7 | |a Aromatic compounds |2 nationallicence | |
| 690 | 7 | |a Comamonas testosteroni CNB-1 |2 nationallicence | |
| 690 | 7 | |a MCP2983 |2 nationallicence | |
| 700 | 1 | |a Ni |D Bin |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Huang |D Zhou |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Wu |D Yu-Fan |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Fan |D Zheng |u Core Facility at Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Jiang |D Cheng-Ying |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Liu |D Shuang-Jiang |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2773-2781 |x 0175-7598 |q 99:6<2773 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6216-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6216-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ni |D Bin |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huang |D Zhou |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Yu-Fan |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fan |D Zheng |u Core Facility at Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jiang |D Cheng-Ying |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Shuang-Jiang |u State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2773-2781 |x 0175-7598 |q 99:6<2773 |1 2015 |2 99 |o 253 | ||