caa a22 4500 605498970 CHVBK 20210128100549.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6216-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6216-3 A novel chemoreceptor MCP2983 from Comamonas testosteroni specifically binds to cis -aconitate and triggers chemotaxis towards diverse organic compounds [Elektronische Daten] [Bin Ni, Zhou Huang, Yu-Fan Wu, Zheng Fan, Cheng-Ying Jiang, Shuang-Jiang Liu] Comamonas testosteroni CNB-1 behaves chemotactically toward a wide range of organic compounds, and 19 methyl-accepting chemotaxis proteins (MCPs) were annotated from the genome of strain CNB-2, a plasmid-curing derivative from strain CNB-1. The MCP-free mutant CNB-1Δ20 completely lost its chemotactic responses. In this study, we found that a chemoreceptor, namely MCP2983, restored chemotactic responses toward nine carboxylic acids and ten aromatic compounds to CNB-1Δ20. Isothermal titration calorimetry analysis indicated that the ligand-binding domain (LBD) of MCP2983 specifically binds to cis-aconitate but not other tested compounds. Deletion of the LBD of MCP2983 impaired chemotactic responses toward cis-aconitate as well as other tested compounds, indicating that the LBD of MCP2983 was essential for triggering chemotactic responses. Five amino acid residues (M81, S156, E157, I158, and L159) that are located at a putative ligand-binding pocket were identified to be involved in binding to cis-aconitate. So far, the MCP2983 represents the sole biochemically identified chemoreceptor that specifically binds to cis-aconitate and is able to trigger chemotaxis towards diverse organic compounds. Springer-Verlag Berlin Heidelberg, 2014 Methyl-accepting chemotaxis proteins (MCPs) nationallicence cis -Aconitate nationallicence Chemoreceptor nationallicence Tricarboxylic acid (TCA) cycle nationallicence Aromatic compounds nationallicence Comamonas testosteroni CNB-1 nationallicence MCP2983 nationallicence Ni Bin State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut Huang Zhou State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut Wu Yu-Fan State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut Fan Zheng Core Facility at Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut Jiang Cheng-Ying State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut Liu Shuang-Jiang State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2773-2781 0175-7598 99:6<2773 2015 99 253 https://doi.org/10.1007/s00253-014-6216-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6216-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ni Bin State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Huang Zhou State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Wu Yu-Fan State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Fan Zheng Core Facility at Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Jiang Cheng-Ying State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Liu Shuang-Jiang State Key Laboratory of Microbial Resources, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2773-2781 0175-7598 99:6<2773 2015 99 253