caa a22 4500 605499020 CHVBK 20210128100549.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-015-6453-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6453-0 RETRACTED ARTICLE: Genome sequence and genome mining of a marine-derived antifungal bacterium Streptomyces sp. M10 [Elektronische Daten] [Jingbo Tang, Xingyan Liu, Jian Peng, Yong Tang, Yang Zhang] A marine-derived actinobacteria Streptomyces sp. M10 was identified as a prolific antifungal compounds producer and shared a 99.02% 16S ribosomal RNA (rRNA) sequence similarity with that of Streptomyces marokkonensis Ap1T, which can produce polyene macrolides. To further evaluate its biosynthetic potential, the 7,207,169bp Streptomyces sp. M10 linear chromosome was sequenced and mined for identifiable secondary metabolite-associated gene clusters. A total of 20 secondary metabolite-associated gene clusters were deduced, including three polyketide synthases (PKSs), four non-ribosomal peptide synthetases (NRPSs), four hybrid NRPS-PKSs, three NRPS-independent siderophores, and two lantibiotic and four terpene biosynthetic gene clusters. One of the type I PKS gene cluster, pks1, shared a 85% nucleotide similarity with candicidin/FR008 gene cluster, indicating the capacity of this organism to produce polyene macrolides. This assumption was verified by a scale-up culturing of Streptomyces sp. M10 on A1 agar plates, which lead to the isolation of two polyene families PF1 and PF2, with characteristic UV adsorption at 269, 278, and 290nm (PF1) and 363, 386, and 408nm (PF2), respectively. Compound 9-04 was further purified from PF1, and its chemical structure was partially elucidated to be atypical polyene macrolide by NMR and UV spectrum. This study affirmatively identified Streptomyces sp. M10 as a source of polyene metabolites and highlighted genome mining of interested organism as a powerful tool for natural product discovery. Springer-Verlag Berlin Heidelberg, 2015 Streptomyces sp. M10 nationallicence Genome mining nationallicence Secondary metabolites nationallicence Polyketide synthase nationallicence Polyene nationallicence Tang Jingbo Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, 410008, Changsha, China aut Liu Xingyan China-America Cancer Research Institute, Guangdong Medical College, Dongguan, China aut Peng Jian Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, 410008, Changsha, China aut Tang Yong Institute of Biomedical Engineering, Xiangya School of Medicine, Central South University, Changsha, China aut Zhang Yang Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, 410008, Changsha, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2763-2772 0175-7598 99:6<2763 2015 99 253 https://doi.org/10.1007/s00253-015-6453-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6453-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang Jingbo Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, 410008, Changsha, China aut NATIONALLICENCE 700 1- Liu Xingyan China-America Cancer Research Institute, Guangdong Medical College, Dongguan, China aut NATIONALLICENCE 700 1- Peng Jian Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, 410008, Changsha, China aut NATIONALLICENCE 700 1- Tang Yong Institute of Biomedical Engineering, Xiangya School of Medicine, Central South University, Changsha, China aut NATIONALLICENCE 700 1- Zhang Yang Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital, Central South University, 410008, Changsha, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2763-2772 0175-7598 99:6<2763 2015 99 253