Beneficial knockouts in Escherichia coli for producing hydrogen from glycerol
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6338-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6338-7 | ||
| 245 | 0 | 0 | |a Beneficial knockouts in Escherichia coli for producing hydrogen from glycerol |h [Elektronische Daten] |c [Kien Tran, Toshinari Maeda, Viviana Sanchez-Torres, Thomas Wood] |
| 520 | 3 | |a Glycerol is an inexpensive and abundant source for biofuel production on a large scale. Escherichia coli is a robust bacterium for producing hydrogen; however, its hydrogen productivity from glycerol is low. In this study, we conducted random transposon mutagenesis to identify uncharacterized genes whose inactivation is beneficial for hydrogen production from glycerol. Through screening, four mutant strains were found that are able to have from 1.3- to 1.6-fold higher hydrogen productivity (μmol H2/mg protein) than that of their parent strain (p < 0.05). These mutations were identified as aroM, gatZ, ycgR, and yfgI. The hydrogen yield (mol H2/mol glycerol consumed) of the aroM, gatZ, ycgR, and yfgI strains was 1.7-, 1.4-, 2.4-, and 2.1-fold higher than that of their parent strain, respectively. Moreover, a single disruption in these genes resulted in a faster cell growth and glycerol consumption under anaerobic conditions. In E. coli, AroM is predicted to be involved in the shikimate pathway, GatZ is tagatose-1,6-bisphosphate aldolase 2 which converts dihydroxyacetone phosphate to 1,6-biphosphate, and YcgR acts as a molecular brake limiting the swimming speed and ATP consumption. So far, the function of YfgI in general and in hydrogen production in particular remains unknown. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Glycerol |2 nationallicence | |
| 690 | 7 | |a Hydrogen production |2 nationallicence | |
| 690 | 7 | |a Escherichia coli |2 nationallicence | |
| 690 | 7 | |a Transposon mutagenesis |2 nationallicence | |
| 700 | 1 | |a Tran |D Kien |u Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Maeda |D Toshinari |u Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan |4 aut | |
| 700 | 1 | |a Sanchez-Torres |D Viviana |u Escuela de Ingeniería Química, Universidad Industrial de Santander, A.A. 678, Bucaramanga, Santander, Colombia |4 aut | |
| 700 | 1 | |a Wood |D Thomas |u Department of Chemical Engineering, Pennsylvania State University, 16802-4400, University Park, PA, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2573-2581 |x 0175-7598 |q 99:6<2573 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6338-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6338-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tran |D Kien |u Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Maeda |D Toshinari |u Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sanchez-Torres |D Viviana |u Escuela de Ingeniería Química, Universidad Industrial de Santander, A.A. 678, Bucaramanga, Santander, Colombia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wood |D Thomas |u Department of Chemical Engineering, Pennsylvania State University, 16802-4400, University Park, PA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2573-2581 |x 0175-7598 |q 99:6<2573 |1 2015 |2 99 |o 253 | ||