caa a22 4500 605499039 CHVBK 20210128100549.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6338-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6338-7 Beneficial knockouts in Escherichia coli for producing hydrogen from glycerol [Elektronische Daten] [Kien Tran, Toshinari Maeda, Viviana Sanchez-Torres, Thomas Wood] Glycerol is an inexpensive and abundant source for biofuel production on a large scale. Escherichia coli is a robust bacterium for producing hydrogen; however, its hydrogen productivity from glycerol is low. In this study, we conducted random transposon mutagenesis to identify uncharacterized genes whose inactivation is beneficial for hydrogen production from glycerol. Through screening, four mutant strains were found that are able to have from 1.3- to 1.6-fold higher hydrogen productivity (μmol H2/mg protein) than that of their parent strain (p < 0.05). These mutations were identified as aroM, gatZ, ycgR, and yfgI. The hydrogen yield (mol H2/mol glycerol consumed) of the aroM, gatZ, ycgR, and yfgI strains was 1.7-, 1.4-, 2.4-, and 2.1-fold higher than that of their parent strain, respectively. Moreover, a single disruption in these genes resulted in a faster cell growth and glycerol consumption under anaerobic conditions. In E. coli, AroM is predicted to be involved in the shikimate pathway, GatZ is tagatose-1,6-bisphosphate aldolase 2 which converts dihydroxyacetone phosphate to 1,6-biphosphate, and YcgR acts as a molecular brake limiting the swimming speed and ATP consumption. So far, the function of YfgI in general and in hydrogen production in particular remains unknown. Springer-Verlag Berlin Heidelberg, 2015 Glycerol nationallicence Hydrogen production nationallicence Escherichia coli nationallicence Transposon mutagenesis nationallicence Tran Kien Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan aut Maeda Toshinari Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan aut Sanchez-Torres Viviana Escuela de Ingeniería Química, Universidad Industrial de Santander, A.A. 678, Bucaramanga, Santander, Colombia aut Wood Thomas Department of Chemical Engineering, Pennsylvania State University, 16802-4400, University Park, PA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2573-2581 0175-7598 99:6<2573 2015 99 253 https://doi.org/10.1007/s00253-014-6338-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6338-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tran Kien Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan aut NATIONALLICENCE 700 1- Maeda Toshinari Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, 808-0196, Kitakyushu, Fukuoka, Japan aut NATIONALLICENCE 700 1- Sanchez-Torres Viviana Escuela de Ingeniería Química, Universidad Industrial de Santander, A.A. 678, Bucaramanga, Santander, Colombia aut NATIONALLICENCE 700 1- Wood Thomas Department of Chemical Engineering, Pennsylvania State University, 16802-4400, University Park, PA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2573-2581 0175-7598 99:6<2573 2015 99 253