Engineering of Escherichia coli for the biosynthesis of poly(3-hydroxybutyrate- co -3-hydroxyhexanoate) from glucose
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6380-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6380-0 | ||
| 245 | 0 | 0 | |a Engineering of Escherichia coli for the biosynthesis of poly(3-hydroxybutyrate- co -3-hydroxyhexanoate) from glucose |h [Elektronische Daten] |c [Qian Wang, Yaqi Luan, Xuelian Cheng, Qianqian Zhuang, Qingsheng Qi] |
| 520 | 3 | |a The copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] has the potential to serve as a biodegradable tissue engineering material. However, the production of this kind of copolymer still suffers from high cost and uncertainty. We describe here the design of metabolic pathways to synthesize P(HB-co-HHx) directly from glucose using recombinant Escherichia coli. By combining the BktB-dependent condensation pathway with the inverted β-oxidation cycle pathway, we were able to synthesize a P(HB-co-HHx) copolymer with a 10mol% HHx fraction in recombinant E. coli. After optimizing the host strain and employing thioesterase mutant strains, the engineered E. coli produced 12.9wt% P(HB-co-HHx) with a 13.2mol% 3HHx fraction. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Escherichia coli |2 nationallicence | |
| 690 | 7 | |a Poly(3-hydroxybutyrate- co -3-hydroxyhexanoate) |2 nationallicence | |
| 690 | 7 | |a Unrelated carbon source |2 nationallicence | |
| 690 | 7 | |a Glucose |2 nationallicence | |
| 690 | 7 | |a BktB-dependent condensation pathway |2 nationallicence | |
| 700 | 1 | |a Wang |D Qian |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | |
| 700 | 1 | |a Luan |D Yaqi |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | |
| 700 | 1 | |a Cheng |D Xuelian |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhuang |D Qianqian |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | |
| 700 | 1 | |a Qi |D Qingsheng |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2593-2602 |x 0175-7598 |q 99:6<2593 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6380-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6380-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Qian |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Luan |D Yaqi |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cheng |D Xuelian |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhuang |D Qianqian |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Qi |D Qingsheng |u National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2593-2602 |x 0175-7598 |q 99:6<2593 |1 2015 |2 99 |o 253 | ||