caa a22 4500 605499047 CHVBK 20210128100549.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-015-6380-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6380-0 Engineering of Escherichia coli for the biosynthesis of poly(3-hydroxybutyrate- co -3-hydroxyhexanoate) from glucose [Elektronische Daten] [Qian Wang, Yaqi Luan, Xuelian Cheng, Qianqian Zhuang, Qingsheng Qi] The copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] has the potential to serve as a biodegradable tissue engineering material. However, the production of this kind of copolymer still suffers from high cost and uncertainty. We describe here the design of metabolic pathways to synthesize P(HB-co-HHx) directly from glucose using recombinant Escherichia coli. By combining the BktB-dependent condensation pathway with the inverted β-oxidation cycle pathway, we were able to synthesize a P(HB-co-HHx) copolymer with a 10mol% HHx fraction in recombinant E. coli. After optimizing the host strain and employing thioesterase mutant strains, the engineered E. coli produced 12.9wt% P(HB-co-HHx) with a 13.2mol% 3HHx fraction. Springer-Verlag Berlin Heidelberg, 2015 Escherichia coli nationallicence Poly(3-hydroxybutyrate- co -3-hydroxyhexanoate) nationallicence Unrelated carbon source nationallicence Glucose nationallicence BktB-dependent condensation pathway nationallicence Wang Qian National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Luan Yaqi National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Cheng Xuelian National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Zhuang Qianqian National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Qi Qingsheng National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2593-2602 0175-7598 99:6<2593 2015 99 253 https://doi.org/10.1007/s00253-015-6380-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6380-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Qian National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Luan Yaqi National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Cheng Xuelian National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Zhuang Qianqian National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Qi Qingsheng National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2593-2602 0175-7598 99:6<2593 2015 99 253