caa a22 4500 605499128 CHVBK 20210128100549.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6176-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6176-7 Application of 16S rRNA metagenomics to analyze bacterial communities at a respiratory care centre in Taiwan [Elektronische Daten] [Chuan Tang, Siu-Ming Yiu, Han-Yueh Kuo, Te-Sheng Tan, Ki-Hok Liao, Chih-Chin Liu, Wing-Kai Hon, Ming-Li Liou] In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectively analyzed during the sampling period in the RCC. A high amount of microbial diversity was detected, with an average of 1,836 phylotypes per sample. In addition to Acinetobacter, more than 60% of the bacterial communities present among the top 25 abundant genera were dominated by skin-associated bacteria. Differences in bacterial profiles were restricted to individual samples. Furthermore, compliance with hand hygiene guidelines may be unsatisfactory among hospital staff according to a principal coordinate analysis that indicated clustering of bacterial communities between devices and workstations for most of the sampling sites. Compared to the high incidence of clinical isolates in the RCC, only Staphylococcus and Acinetobacter were highly abundant in the IHEs. Despite Acinetobacter was the most abundant genus present in IHEs of the RCC, potential pathogens, e.g., Acinetobacter baumannii, might remain susceptible to carbapenem. This study is the first in Taiwan to demonstrate a high diversity of human-associated bacteria in the RCC via 16S rRNA metagenomics, which allows for new assessment of potential health risks in RCCs, aids in the evaluation of existing sanitation protocols, and furthers our understanding of the development of healthcare-associated infections. Springer-Verlag Berlin Heidelberg, 2014 16S rRNA metagenomics nationallicence Healthcare-associated infection nationallicence Acinetobacter nationallicence Respiratory care center nationallicence Tang Chuan Department of Computer Science and Information Engineering, Providence University, Taichung County, Taiwan aut Yiu Siu-Ming Department of Computer Science, The University of Hong Kong, Hong Kong, China aut Kuo Han-Yueh Department of Medicine, National Taiwan University Hospital Hsin-Chu Branch, Hsin-Chu City, Taiwan aut Tan Te-Sheng Department of Computer Science, National Tsing Hua University, Hsin-Chu City, Taiwan aut Liao Ki-Hok Department of Computer Science, National Tsing Hua University, Hsin-Chu City, Taiwan aut Liu Chih-Chin Department of Computer Science and Information Engineering, Providence University, Taichung County, Taiwan aut Hon Wing-Kai Department of Computer Science, National Tsing Hua University, Hsin-Chu City, Taiwan aut Liou Ming-Li Department of Computer Science and Information Engineering, Providence University, Taichung County, Taiwan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2871-2881 0175-7598 99:6<2871 2015 99 253 https://doi.org/10.1007/s00253-014-6176-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6176-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang Chuan Department of Computer Science and Information Engineering, Providence University, Taichung County, Taiwan aut NATIONALLICENCE 700 1- Yiu Siu-Ming Department of Computer Science, The University of Hong Kong, Hong Kong, China aut NATIONALLICENCE 700 1- Kuo Han-Yueh Department of Medicine, National Taiwan University Hospital Hsin-Chu Branch, Hsin-Chu City, Taiwan aut NATIONALLICENCE 700 1- Tan Te-Sheng Department of Computer Science, National Tsing Hua University, Hsin-Chu City, Taiwan aut NATIONALLICENCE 700 1- Liao Ki-Hok Department of Computer Science, National Tsing Hua University, Hsin-Chu City, Taiwan aut NATIONALLICENCE 700 1- Liu Chih-Chin Department of Computer Science and Information Engineering, Providence University, Taichung County, Taiwan aut NATIONALLICENCE 700 1- Hon Wing-Kai Department of Computer Science, National Tsing Hua University, Hsin-Chu City, Taiwan aut NATIONALLICENCE 700 1- Liou Ming-Li Department of Computer Science and Information Engineering, Providence University, Taichung County, Taiwan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2871-2881 0175-7598 99:6<2871 2015 99 253