caa a22 4500 605499152 CHVBK 20210128100549.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6164-y doi (NATIONALLICENCE)springer-10.1007/s00253-014-6164-y Isolation of a novel beta-cypermethrin degrading strain Bacillus subtilis BSF01 and its biodegradation pathway [Elektronische Daten] [Ying Xiao, Shaohua Chen, Yuanqi Gao, Wei Hu, Meiying Hu, Guohua Zhong] Continuous use of the pyrethroid insecticide beta-cypermethrin (beta-cp) has resulted in serious environmental contamination problems. We report here that a novel bacterial strain BSF01, which was isolated from activated sludge and identified as Bacillus subtilis (collection number: CCTCC AB 2014103), showed high efficiency in degrading beta-cp. Strain BSF01 was able to utilize beta-cp as the sole carbon source for growth and degraded 89.4% of 50mgL−1 beta-cp within 7days. The optimal conditions for beta-cp degradation were determined to be 34.5°C, pH 6.7, and inocula amount 0.11g dry wtL−1 using response surface methodology. The kinetic parameters q max, K s, and K i were established to be 2.19day−1, 76.37mgL−1, and 54.14mgL−1, respectively. The critical inhibitor concentration was determined to be 64.30mgL−1. Seven metabolites were identified by gas chromatography-mass spectrometry. Furthermore, a novel biodegradation pathway for beta-cp was proposed on the basis of analysis of the metabolites. This strain was also capable of degrading a wide range of pyrethroid insecticides including cypermethrin, deltamethrin, cyhalothrin, and beta-cyfluthrin, which similar to beta-cp are hazardous chemicals. Taken together, our results depict the biodegradation pathway of beta-cp and highlight the promising potentials of strain BSF01 in bioremediation of pyrethroid-contaminated environments. Springer-Verlag Berlin Heidelberg, 2014 Bioremediation nationallicence Beta-cypermethrin nationallicence Bacillus subtilis BSF01 nationallicence Metabolites nationallicence Biodegradation pathway nationallicence Xiao Ying Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Chen Shaohua Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Gao Yuanqi Guangdong Tianhe Agricultural Means of Production Co., Ltd., Guangzhou, 510642, People's Republic of China aut Hu Wei Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Hu Meiying Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Zhong Guohua Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2849-2859 0175-7598 99:6<2849 2015 99 253 https://doi.org/10.1007/s00253-014-6164-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6164-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiao Ying Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Shaohua Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Gao Yuanqi Guangdong Tianhe Agricultural Means of Production Co., Ltd., Guangzhou, 510642, People's Republic of China aut NATIONALLICENCE 700 1- Hu Wei Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Meiying Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhong Guohua Laboratory of Insect Toxicology, and Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, 510642, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2849-2859 0175-7598 99:6<2849 2015 99 253