Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10
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| 008 | 210128e20150301xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6307-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6307-1 | ||
| 245 | 0 | 0 | |a Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10 |h [Elektronische Daten] |c [Shui-Ping Liu, Pin Yu, Peng-Hui Yuan, Zhen-Xing Zhou, Qing-Ting Bu, Xu-Ming Mao, Yong-Quan Li] |
| 520 | 3 | |a The roles of many sigma factors are unclear in regulatory mechanism of secondary metabolism in Streptomyces. Here, we report the regulation network of a group 3 sigma factor, WhiGch, from a natamycin industrial strain Streptomyces chattanoogensis L10. WhiGch regulates the growth and morphological differentiation of S. chattanoogensis L10. The whiG ch deletion mutant decreased natamycin production by about 30% and delayed natamycin production more than 24h by delaying the growth. Overexpression of the whiG ch gene increased natamycin production in large scale production medium by about 26%. WhiGch upregulated the transcription of natamycin biosynthetic gene cluster and inhibited the expression of migrastatin and jadomycin analog biosynthetic polyketide synthase genes. WhiGch positively regulated natamycin biosynthetic gene cluster by directly binding to the promoters of scnC and scnD, which were involved in natamycin biosynthesis, and these binding sites adjacent to translation start codon were determined. Thus, this paper further elucidates the high natamycin yield mechanisms of industrial strains and demonstrates that a valuable improvement in the yield of the target metabolites can be achieved through manipulating the transcription regulators. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Sigma factor |2 nationallicence | |
| 690 | 7 | |a Morphological differentiation |2 nationallicence | |
| 690 | 7 | |a Secondary metabolism |2 nationallicence | |
| 690 | 7 | |a Streptomyces |2 nationallicence | |
| 700 | 1 | |a Liu |D Shui-Ping |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Yu |D Pin |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Yuan |D Peng-Hui |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Zhou |D Zhen-Xing |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Bu |D Qing-Ting |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Mao |D Xu-Ming |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | |
| 700 | 1 | |a Li |D Yong-Quan |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2715-2726 |x 0175-7598 |q 99:6<2715 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6307-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6307-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Shui-Ping |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Pin |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yuan |D Peng-Hui |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Zhen-Xing |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bu |D Qing-Ting |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mao |D Xu-Ming |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Yong-Quan |u College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2715-2726 |x 0175-7598 |q 99:6<2715 |1 2015 |2 99 |o 253 | ||