caa a22 4500 605499209 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6307-1 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6307-1 Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10 [Elektronische Daten] [Shui-Ping Liu, Pin Yu, Peng-Hui Yuan, Zhen-Xing Zhou, Qing-Ting Bu, Xu-Ming Mao, Yong-Quan Li] The roles of many sigma factors are unclear in regulatory mechanism of secondary metabolism in Streptomyces. Here, we report the regulation network of a group 3 sigma factor, WhiGch, from a natamycin industrial strain Streptomyces chattanoogensis L10. WhiGch regulates the growth and morphological differentiation of S. chattanoogensis L10. The whiG ch deletion mutant decreased natamycin production by about 30% and delayed natamycin production more than 24h by delaying the growth. Overexpression of the whiG ch gene increased natamycin production in large scale production medium by about 26%. WhiGch upregulated the transcription of natamycin biosynthetic gene cluster and inhibited the expression of migrastatin and jadomycin analog biosynthetic polyketide synthase genes. WhiGch positively regulated natamycin biosynthetic gene cluster by directly binding to the promoters of scnC and scnD, which were involved in natamycin biosynthesis, and these binding sites adjacent to translation start codon were determined. Thus, this paper further elucidates the high natamycin yield mechanisms of industrial strains and demonstrates that a valuable improvement in the yield of the target metabolites can be achieved through manipulating the transcription regulators. Springer-Verlag Berlin Heidelberg, 2015 Sigma factor nationallicence Morphological differentiation nationallicence Secondary metabolism nationallicence Streptomyces nationallicence Liu Shui-Ping College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut Yu Pin College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut Yuan Peng-Hui College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut Zhou Zhen-Xing College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut Bu Qing-Ting College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut Mao Xu-Ming College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut Li Yong-Quan College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2715-2726 0175-7598 99:6<2715 2015 99 253 https://doi.org/10.1007/s00253-014-6307-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6307-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Shui-Ping College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Yu Pin College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Yuan Peng-Hui College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Zhou Zhen-Xing College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Bu Qing-Ting College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Mao Xu-Ming College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Li Yong-Quan College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2715-2726 0175-7598 99:6<2715 2015 99 253