caa a22 4500 605499217 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-015-6403-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6403-x Guidelines for development and implementation of biocatalytic P450 processes [Elektronische Daten] [Marie Lundemo, John Woodley] Biocatalytic reactions performed by cytochrome P450 monooxygenases are interesting in pharmaceutical research since they are involved in human drug metabolism. Furthermore, they are potentially interesting as biocatalysts for synthetic chemistry because of the exquisite selectivity of the chemistry they undertake. For example, selective hydroxylation can be undertaken on a highly functionalized molecule without the need for functional group protection. Recent progress in the discovery of novel P450s as well as protein engineering of these enzymes strongly encourages further development of their application, including use in synthetic processes. The biological characteristics of P450s (e.g., cofactor dependence) motivate the use of whole-cell systems for synthetic processes, and those processes implemented in industry are so far dominated by growing cells and native host systems. However, for an economically feasible process, the expression of P450 systems in a heterologous host with sufficient biocatalyst yield (g/g cdw) for non-growing systems or space-time yield (g/L/h) for growing systems remains a major challenge. This review summarizes the opportunities to improve P450 whole-cell processes and strategies in order to apply and implement them in industrial processes, both from a biological and process perspective. Indeed, a combined approach of host selection and cell engineering, integrated with process engineering, is suggested as the most effective route to implementation. Springer-Verlag Berlin Heidelberg, 2015 Biocatalysis nationallicence P450 monooxygenases nationallicence Whole cell biotransformation nationallicence Bioprocess engineering nationallicence Lundemo Marie Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800, Kgs. Lyngby, Denmark aut Woodley John Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800, Kgs. Lyngby, Denmark aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2465-2483 0175-7598 99:6<2465 2015 99 253 https://doi.org/10.1007/s00253-015-6403-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6403-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lundemo Marie Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Woodley John Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2465-2483 0175-7598 99:6<2465 2015 99 253