caa a22 4500 605499225 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6278-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6278-2 Plasma membrane proteins Yro2 and Mrh1 are required for acetic acid tolerance in Saccharomyces cerevisiae [Elektronische Daten] [Akiko Takabatake, Nozomi Kawazoe, Shingo Izawa] Yro2 and its paralogous protein Mrh1 of Saccharomyces cerevisiae have seven predicted transmembrane domains and predominantly localize to the plasma membrane. Their physiological functions and regulation of gene expression have not yet been elucidated in detail. We herein demonstrated that MRH1 was constitutively expressed, whereas the expression of YRO2 was induced by acetic acid stress and entering the stationary phase. Fluorescence microscopic analysis revealed that Mrh1 and Yro2 were distributed as small foci in the plasma membrane under acetic acid stress conditions. The null mutants of these genes (mrh1∆, yro2∆, and mrh1∆yro2∆) showed delayed growth and a decrease in the productivity of ethanol in the presence of acetic acid, indicating that Yro2 and Mrh1 are involved in tolerance to acetic acid stress. Springer-Verlag Berlin Heidelberg, 2014 Acetic acid stress nationallicence Bioethanol nationallicence Lignocellulosic biomass nationallicence Haa1 nationallicence Saccharomyces cerevisiae nationallicence Takabatake Akiko Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, 606-8585, Kyoto, Japan aut Kawazoe Nozomi Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, 606-8585, Kyoto, Japan aut Izawa Shingo Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, 606-8585, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2805-2814 0175-7598 99:6<2805 2015 99 253 https://doi.org/10.1007/s00253-014-6278-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6278-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takabatake Akiko Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, 606-8585, Kyoto, Japan aut NATIONALLICENCE 700 1- Kawazoe Nozomi Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, 606-8585, Kyoto, Japan aut NATIONALLICENCE 700 1- Izawa Shingo Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, 606-8585, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2805-2814 0175-7598 99:6<2805 2015 99 253