caa a22 4500 605499241 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6312-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6312-4 One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase [Elektronische Daten] [Julia Marín-Navarro, David Talens-Perales, Julio Polaina] We describe a simple, efficient process for the production of 6-kestose, a trisaccharide with well-documented prebiotic properties. A key factor is the use of a yeast transformant expressing an engineered version of Saccharomyces invertase with enhanced transfructosylating activity. When the yeast transformant was grown with 30% sucrose as the carbon source, 6-kestose accumulated up to ca. 100g/L in the culture medium. The 6-kestose yield was significantly enhanced (up to 200g/L) using a two-stage process carried out in the same flask. In the first stage, the culture was grown in 30% sucrose at physiological temperature (30°C) to allow overexpression of the invertase. In the second stage, sucrose was added to the culture at high concentration (60%) and the temperature shifted to 50°C. In both cases, 6-kestose was synthesized with high specificity, representing more than 95% of total FOS. Springer-Verlag Berlin Heidelberg, 2014 Cell factory nationallicence Kestose nationallicence Prebiotics nationallicence Sucrose nationallicence Transglycosylation nationallicence Marín-Navarro Julia Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut Talens-Perales David Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut Polaina Julio Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2549-2555 0175-7598 99:6<2549 2015 99 253 https://doi.org/10.1007/s00253-014-6312-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6312-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marín-Navarro Julia Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut NATIONALLICENCE 700 1- Talens-Perales David Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut NATIONALLICENCE 700 1- Polaina Julio Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2549-2555 0175-7598 99:6<2549 2015 99 253