caa a22 4500 60549925X CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6075-y doi (NATIONALLICENCE)springer-10.1007/s00253-014-6075-y Exopolysaccharide biosynthesis and biodegradation by a marine hydrothermal Alteromonas sp [Elektronische Daten] strain [Florian Lelchat, Joelle Cozien, Tinaïg Le Costaouec, Christophe Brandilly, Sophie Schmitt, Anne-Claire Baudoux, Sylvia Colliec-Jouault, Claire Boisset] Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they specifically cleave glycosidic bonds and do not require harsh chemical conditions. The HYD657 EPS is structurally complex and no commercially available enzymes are able to effectively degrade it. Here, we present the first results on the endogenous enzymatic depolymerization of a marine EPS of biotechnological interest by the producing strain. Enzymatic activity was detected in the bacterial lysate and was able to decrease the apparent molecular size of the EPS, releasing mainly oligosaccharides. The reduced form of the native polysaccharide showed a slightly modified osidic composition, particularly in terms of molar ratio. Several exoglycosidase activities were measured in the bacterial lysate using paranitrophenyl-osides. Springer-Verlag Berlin Heidelberg, 2014 Marine Alteromonas macleodii sp . strain nationallicence Bacterial exopolysaccharides nationallicence Microbial extracellular enzymes nationallicence Endogenous enzymatic degradation nationallicence Lelchat Florian IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut Cozien Joelle IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut Le Costaouec Tinaïg CERMAV, 601 rue de la Chimie, 38049, Grenoble cedex 9, France aut Brandilly Christophe IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut Schmitt Sophie IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut Baudoux Anne-Claire Station Biologique de Roscoff, CNRS, 29680, Roscoff, France aut Colliec-Jouault Sylvia IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut Boisset Claire IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2637-2647 0175-7598 99:6<2637 2015 99 253 https://doi.org/10.1007/s00253-014-6075-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6075-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lelchat Florian IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Cozien Joelle IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Le Costaouec Tinaïg CERMAV, 601 rue de la Chimie, 38049, Grenoble cedex 9, France aut NATIONALLICENCE 700 1- Brandilly Christophe IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Schmitt Sophie IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Baudoux Anne-Claire Station Biologique de Roscoff, CNRS, 29680, Roscoff, France aut NATIONALLICENCE 700 1- Colliec-Jouault Sylvia IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut NATIONALLICENCE 700 1- Boisset Claire IFREMER, Technopole Brest Iroise, 29280, Plouzané, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2637-2647 0175-7598 99:6<2637 2015 99 253