caa a22 4500 605499268 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6257-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6257-7 Development of Lactobacillus paracasei harboring nucleic acid-hydrolyzing 3D8 scFv as a preventive probiotic against murine norovirus infection [Elektronische Daten] [Phuong Hoang, Seungchan Cho, Kee Kim, Sung Byun, Taek-Kyun Lee, Sukchan Lee] The protein 3D8 single-chain variable fragment (3D8 scFv) has potential anti-viral activity due to its ability to penetrate into cells and hydrolyze nucleic acids. Probiotic Lactobacillus paracasei engineered to secrete 3D8 scFv for oral administration was used to test the anti-viral effects of 3D8 scFv against gastrointestinal virus infections. We found that injection of 3D8 scFv into the intestinal lumen resulted in the penetration of 3D8 scFv into the intestinal villi and lamina propria. 3D8 scFv secreted from engineered L. paracasei retained its cell-penetrating and nucleic acid-hydrolyzing activities, which were previously shown with 3D8 scFv expressed in Escherichia coli. Pretreatment of RAW264.7 cells with 3D8 scFv purified from L. paracasei prevented apoptosis induction by murine norovirus infection and decreased messenger RNA (mRNA) expression of the viral capsid protein VP1. In a mouse model, oral administration of the engineered L. paracasei prior to murine norovirus infection reduced the expression level of mRNA encoding viral polymerase. Taken together, these results suggest that L. paracasei secreting 3D8 scFv provides a basis for the development of ingestible anti-viral probiotics active against gastrointestinal viral infection. Springer-Verlag Berlin Heidelberg, 2014 3D8 scFv nationallicence Anti-viral nationallicence Lactobacillus paracasei nationallicence Oral administration nationallicence Murine norovirus nationallicence Hoang Phuong Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut Cho Seungchan Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut Kim Kee Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut Byun Sung Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, 143-13 Seosuwonro, 441-706, Gwonsun-gu, Suwon, Korea aut Lee Taek-Kyun South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, 656-830, Geoje, Korea aut Lee Sukchan Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2793-2803 0175-7598 99:6<2793 2015 99 253 https://doi.org/10.1007/s00253-014-6257-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6257-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hoang Phuong Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut NATIONALLICENCE 700 1- Cho Seungchan Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut NATIONALLICENCE 700 1- Kim Kee Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut NATIONALLICENCE 700 1- Byun Sung Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, 143-13 Seosuwonro, 441-706, Gwonsun-gu, Suwon, Korea aut NATIONALLICENCE 700 1- Lee Taek-Kyun South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, 656-830, Geoje, Korea aut NATIONALLICENCE 700 1- Lee Sukchan Department of Genetic Engineering, Sungkyunkwan University, 2066 Seobu-ro, 440-746, Jangan-gu, Suwon, Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2793-2803 0175-7598 99:6<2793 2015 99 253