Capturing the target genes of BldD in Saccharopolyspora erythraea using improved genomic SELEX method
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6255-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6255-9 | ||
| 245 | 0 | 0 | |a Capturing the target genes of BldD in Saccharopolyspora erythraea using improved genomic SELEX method |h [Elektronische Daten] |c [Hang Wu, Yongrong Mao, Meng Chen, Hui Pan, Xunduan Huang, Min Ren, Hao Wu, Jiali Li, Zhongdong Xu, Hualing Yuan, Ming Geng, David Weaver, Lixin Zhang, Buchang Zhang] |
| 520 | 3 | |a BldD (SACE_2077), a key developmental regulator in actinomycetes, is the first identified transcriptional factor in Saccharopolyspora erythraea positively regulating erythromycin production and morphological differentiation. Although the BldD of S. erythraea binds to the promoters of erythromycin biosynthetic genes, the interaction affinities are relatively low, implying the existence of its other target genes in S. erythraea. Through the genomic systematic evolution of ligands by exponential enrichment (SELEX) method that we herein improved, four DNA sequences of S. erythraea A226, corresponding to the promoter regions of SACE_0306 (beta-galactosidase), SACE_0811 (50S ribosomal protein L25), SACE_3410 (fumarylacetoacetate hydrolase), and SACE_6014 (aldehyde dehydrogenase), were captured with all three BldD concentrations of 0.5, 1, and 2μM, while the previously identified intergenic regions of eryBIV-eryAI and ermE-eryCI plus the promoter region of SACE_7115, the amfC homolog for aerial mycelium formation, could be captured only when the BldD's concentration reached 2μM. Electrophoretic mobility shift assay (EMSA) analysis indicated that BldD specifically bound to above seven DNA sequences, and quantitative real-time PCR (qRT-PCR) assay showed that the transcriptional levels of the abovementioned target genes decreased when bldD was disrupted in A226. Furthermore, SACE_7115 and SACE_0306 in A226 were individually inactivated, showing that SACE_7115 was predominantly involved in aerial mycelium formation, while SACE_0306 mainly controlled erythromycin production. This study provides valuable information for better understanding of the pleiotropic regulator BldD in S. erythraea, and the improved method may be useful for uncovering regulatory networks of other transcriptional factors. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Saccharopolyspora erythraea |2 nationallicence | |
| 690 | 7 | |a Improved genomic SELEX method |2 nationallicence | |
| 690 | 7 | |a BldD |2 nationallicence | |
| 690 | 7 | |a amfC |2 nationallicence | |
| 690 | 7 | |a SACE_0306 |2 nationallicence | |
| 700 | 1 | |a Wu |D Hang |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Mao |D Yongrong |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Chen |D Meng |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Pan |D Hui |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Huang |D Xunduan |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Ren |D Min |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Wu |D Hao |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Li |D Jiali |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Xu |D Zhongdong |u School of Life Sciences, Hefei Normal University, 234000, Hefei, China |4 aut | |
| 700 | 1 | |a Yuan |D Hualing |u School of Life Sciences, Hefei Normal University, 234000, Hefei, China |4 aut | |
| 700 | 1 | |a Geng |D Ming |u School of Life Sciences, Hefei Normal University, 234000, Hefei, China |4 aut | |
| 700 | 1 | |a Weaver |D David |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Zhang |D Lixin |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Zhang |D Buchang |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2683-2692 |x 0175-7598 |q 99:6<2683 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6255-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6255-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Hang |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mao |D Yongrong |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Meng |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pan |D Hui |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huang |D Xunduan |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ren |D Min |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Hao |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Jiali |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Zhongdong |u School of Life Sciences, Hefei Normal University, 234000, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yuan |D Hualing |u School of Life Sciences, Hefei Normal University, 234000, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Geng |D Ming |u School of Life Sciences, Hefei Normal University, 234000, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Weaver |D David |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Lixin |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Buchang |u Institute of Health Sciences, School of Life Sciences, Anhui University, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/6(2015-03-01), 2683-2692 |x 0175-7598 |q 99:6<2683 |1 2015 |2 99 |o 253 | ||