caa a22 4500 605499284 CHVBK 20210128100550.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6187-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6187-4 Primary effects of extracellular enzyme activity and microbial community on carbon and nitrogen mineralization in estuarine and tidal wetlands [Elektronische Daten] [Xiaofei Li, Lijun Hou, Min Liu, Xianbiao Lin, Ye Li, Shuwen Li] Estuarine and tidal wetlands with high primary productivity and biological activity play a crucial role in coastal nutrient dynamics. Here, to better reveal the effects of extracellular enzymes and microbial community on carbon (C) and nitrogen (N) mineralization, the incubation experiments with different C and N addition patterns to the tidal sediments of the Yangtze Estuary (China) were conducted. The results suggested a significant increase in cumulative CO2 effluxes in the C and CN treatment experiments, while no significant difference in cumulative CO2 effluxes between the N treatment and control (CK) experiments was observed. In addition, the nutrient addition patterns had a great influence on dissolve organic C and N levels, but a small effect on microbial biomass C and N. Microbial community composition and microbial activity were found to be positively correlated with organic C (OC) and the molar ratio of C to N (C/N). Partial correlation analysis, controlling for C/N, supported direct effects of OC on the activity of carbon-cycling extracellular enzymes (cellulase and polyphenol oxidase), while C/N exhibited negatively correlations with urease and Gram-positive bacteria to Gram-negative bacteria (G+/G−). Strong relationships were found between CO2 efflux and mineral nitrogen with the activity of specific enzymes (sucrase, cellulase, and polyphenol oxidase) and abundances of Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungi, suggesting the significant influences of microbial community and enzyme activity on C and N mineralization in the estuarine and tidal wetlands. Furthermore, this study could highlight the need to explore effects of nutrient supply on microbial communities and enzyme activity changes associated with the C and N mineralization in these wetlands induced by the climate change. Springer-Verlag Berlin Heidelberg, 2014 Extracellular enzyme activity nationallicence Microbial community composition nationallicence Phospholipid fatty acid (PLFA) nationallicence C and N mineralization nationallicence Estuarine and tidal wetlands nationallicence Yangtze estuary nationallicence Li Xiaofei College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Hou Lijun State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Liu Min College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Lin Xianbiao College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Li Ye College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Li Shuwen College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2895-2909 0175-7598 99:6<2895 2015 99 253 https://doi.org/10.1007/s00253-014-6187-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6187-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xiaofei College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Hou Lijun State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Liu Min College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Lin Xianbiao College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Li Ye College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Li Shuwen College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/6(2015-03-01), 2895-2909 0175-7598 99:6<2895 2015 99 253